CFDA（ 5-羧基荧光素二乙酸酯）

5-羧基荧光素二乙酸酯  (5-CFDA，5-Carboxyfluorescein diacetate)是一种可渗入细胞并被水解为5-carboxyfluorescein，用于标记人椎间盘细胞以便体外荧光显微镜观察。荧光 :λex 290± 3 nm in methanol; λex 492± 3 nm, λem 520 ± 4 nm in Buffer pH 9。

溶解度SolubilitySoluble in DMSO (5 mg/ml), DMF, chloroform, Acetonitrile, and 1-2% Methanol/Chloroform.

性状：solid

储藏条件：储存温度2-8°C

5- 羧基荧光素二乙酸酯5-CFDA 应用：
5-Carboxyfluorescein diacetate is a vital stain. Non-fluorescent, hydrophobic agent that permeates intact cell membranes. In the cell, 5-Carboxyfluorescein diacetate is enzymatically hydrolyzed to a hydrophilic agent, strongly fluorescent 5-Carboxyfluorescein.

5-CFDA 案例：

Images showing CFDA absorption into the adaxial leaf surface and symplastic movement of CF into the epidermal cells of the abaxial side. ( a ) A confocal image of the adaxial leaf surface demonstrating proper dye absorption after 5 min of loading. ( b ) A close-up confocal image showing dye absorption into mature guard cells as well as pavement cells. ( c ) A confocal image of the abaxial leaf surface showing proper dye diffusion into epidermal cells of the abaxial side of the leaf after 5 min of loading of CFDA on the adaxial leaf surface. ( d ) A close-up confocal image showing symplastic movement of CF in the abaxial leaf epidermis based on the lack of CF fl uorescence in mature guard cells. ( a ) and ( c ) are merged images of green (for CF) and red (for chloroplast autofl uorescence) channels. Darts , mature guard cells. Size bars = 20 μm in ( a ) and ( c ) and 200 μm in ( b ) and ( d ). Images are adapted from Lee et al.

Cell staining mechanism