通过监测多个诊断化学标记物来鉴定和区分人参，西洋参和三七

通过监测多个诊断化学标记物来鉴定和区分人参，西洋参和三七。
Acta Pharmaceutica Sinica B ( IF 7.097 ) Pub Date : 2016-11-08 , DOI: 10.1016/j.apsb.2016.05.005
Wenzhi Yang,Xue Qiao,Kai Li,Jingran Fan,Tao Bo,De-An Guo,Min Ye

在中药复方制剂中区分同属物种的中药（TCM）通常具有挑战性。人参（PG），西洋参（PQ）和三七（PN）的根用作流行的中药。它们含有类似的三萜皂苷（人参皂苷）作为主要的生物活性成分。迄今为止，仅发现了一些化学标记来区分这三个物种。在本文中，我们提出了一种多标记物检测方法，以有效地区分三种人参物种，并在化合物制剂中进行鉴定。首先，使用经过验证的LC-MS指纹图谱方法，通过监测提取的离子色谱图（EIC）中的40种主要人参皂苷，对85批次的粗制药物样品（包括32 PG，30 PQ和23 PN）进行了分析。其次，使用PLS-DA和OPLS-DA模型，通过模式识别化学计量学方法将样品分为不同的组，并发现了17种诊断化学标记。除了先前已知的Rf和p-F11，人参皂苷Rs1可能是区分PG和PQ的新标记。最后，使用上述多种化学标记物鉴定了60批次中药复方制剂中的人参种类。

Identification and differentiation of Panax ginseng, Panax quinquefolium, and Panax notoginseng by monitoring multiple diagnostic chemical markers.

To differentiate traditional Chinese medicines (TCM) derived from congeneric species in TCM compound preparations is usually challenging. The roots of Panax ginseng (PG), Panax quinquefolium (PQ) and Panax notoginseng (PN) are used as popular TCM. They contain similar triterpenoid saponins (ginsenosides) as the major bioactive constituents. Thus far, only a few chemical markers have been discovered to differentiate these three species. Herein we present a multiple marker detection approach to effectively differentiate the three Panax species, and to identify them in compound preparations. Firstly, 85 batches of crude drug samples (including 32 PG, 30 PQ, and 23 PN) were analyzed by monitoring 40 major ginsenosides in the extracted ion chromatograms (EICs) using a validated LC-MS fingerprinting method. Secondly, the samples were clustered into different groups by pattern recognition chemometric approaches using PLS-DA and OPLS-DA models, and 17 diagnostic chemical markers were discovered. Aside from the previously known Rf and p-F11, ginsenoside Rs1 could be a new marker to differentiate PG from PQ. Finally, the above multiple chemical markers were used to identify the Panax species in 60 batches of TCM compound preparations.