How to replace the sample needle on an ACQUITY Flow Through Needle (FTN) Sample Manager - WKB7319

如何更换穿针取样管上的取样针

OBJECTIVE or GOAL
目的或目标
Replace the sample needle on an FTN.
更换 FTN 上的样品针。

ENVIRONMENT
环境

• ACQUITY UPLC H-Class FTN Sample Manager H 级 FTN 样品管理器

• ACQUITY UPLC I-Class FTN Sample Manager 一级 FTN 样品管理器

PROCEDURE
程序

1. In the Console, select the Sample Manager-FTN from the system tree. 在控制台中，从系统树中选择 Sample Manager-FTN

2. Click Maintain > Replace > Needle. 点击维护 > 更换 > 针头

3. Open the sample compartment door. 打开样品室的门

4. Using a T20 TORX driver, loosen the two captive screws that secure the access panel, and then remove the panel. 使用 T20 TORX 驱动程序，松开两个圈养螺丝固定访问面板，然后删除面板

1. Remove the sample plate if a plate is loaded. 如果加载了样板，则拆卸样板

2. Unscrew the needle assembly's fitting from injector port 4. 从注射器口4拧下针头总成的配件

1. Inside the sample compartment, open the needle-tubing guide cover, located on the roof of the compartment, by rotating clockwise. 在样品室内，打开位于样品室顶部的针管导向盖，顺时针旋转

1. Push the needle latch back to release the needle mounting cylinder from its mounting cavity, and the needle tubing from its notches. 推动针锁存回来，释放针安装缸从其安装腔，针管从其缺口

1. Lift the needle tip out of the needle guide at the bottom of the needle mechanism. 将针尖从针头机构底部的针头导轨上提起

Warning: To avoid puncture wounds or damage to the end of the needle, do not touch or press the end of the sample needle.
警告: 为了避免穿刺伤口或针头末端的损坏，请不要触摸或按压样品针头的末端。

1. Using a T10 TORX driver, loosen the screw on the needle-tubing clamp and remove the tubing. 使用 T10 TORX 驱动程序，松开针管夹上的螺丝，并删除油管

1. Using a T6 TORX driver, loosen the needle guide set screw, and then remove the needle guide. 使用 T6 TORX 驱动程序，松开针引导设置螺丝，然后删除针引导

1. Install the new needle guide and tighten the set screw. 安装新的针头导轨，拧紧固定螺丝

2. Holding the new needle assembly with both hands to control its position within the sample compartment, secure the mounting sleeve in the opening of the tubing clamp, with the fitting oriented toward the valve. Caution: To avoid letting go of the needle assembly and possibly damaging the tip of the needle, do not tighten the clamp screw during this step of the procedure. 用双手握住新的针组件，以控制其在样品室内的位置，将安装套管固定在油管夹的开口处，使安装套管朝向阀门。注意: 为了避免松开针组件并可能损坏针头的尖端，不要在此过程中拧紧夹紧螺丝

1. From inside the sample compartment, take hold of the needle assembly tubing as it enters the compartment from above. 从内部的样品隔间，抓住针装配管，因为它进入隔间从上面

2. Ensure that the needle tubing is routed to the left side of the needle carriage's rails and is secured in the guide channel on the roof of the compartment. 确保针管路由到针车轨道的左侧，并固定在隔间顶部的导向通道中

1. Remove the protective cap from the needle tip. 从针尖上取下保护帽

2. Insert the needle mounting cylinder into the mounting cavity. 将安装针筒插入安装腔内

Caution: To avoid damaging the instrument, do not place the loose loop of tubing above the Z-flag.
警告: 为了避免损坏仪器，不要将松动的管路环置于 z 标志之上。

1. Route the tubing through the two notches below the Z-flag. 将油管穿过 z 旗下的两个凹槽

2. Push the needle latch forward to secure the needle assembly. 向前推针锁存器以固定针总成

1. Using a T10 TORX driver, tighten the screw on the needle-tubing clamp. 使用 T10 TORX 驱动程序，拧紧针管夹上的螺丝

2. Insert the needle's tip into the needle guide at the bottom of the needle mechanism. 将针头插入针头机构底部的针头导轨中

1. Ensure that the needle tubing is fully inserted into port 4 on the injection valve, and then thread the fitting into the port, tightening the fitting securely. 确保针管完全插入注射阀的4口，然后将接头螺纹固定在4口，紧固接头

2. Reinstall the access panel using the T20 TORX driver to tighten the two screws that secure it to the front of the unit. 使用 T20 TORX 驱动程序重新安装访问面板，拧紧固定在机组前部的两个螺丝

Requirement: Ensure that the seal port tube is routed under the gap on the side of the access panel.
要求: 确保密封端口管路由在接入面板侧面的间隙之下。

1. Close the sample compartment and fluidics compartment doors. 关闭样品隔间和流体隔间门

2. Calibrate the needle's 校准指针z axis (see the ACQUITY UPLC online Help). 轴(请参阅 ACQUITY UPLC 在线帮助)

• See article: 参见文章:How to calibrate the needle Z axis - WKB28509 如何校准针头 z 轴 -WKB28509

1. Characterize the needle seal (see the ACQUITY UPLC online Help). 描述针密封(请参阅 ACQUITY UPLC 在线帮助)

2. Perform the needle seal readiness test (see the ACQUITY UPLC online Help). 执行针密封准备测试(请参阅 ACQUITY UPLC 在线帮助)

• See Video:

Diagnostics: Needle-Seal Readiness Testsite-436448.bcvp0rtal.com/detail/videos/how-to-series/video/4334162061001/diagnostics:-needle-seal-readiness