源码阅读网spRing Boot 急行军
java -jar $GATK \
-T HaplotypeCaller \
-R $DB/hg19.fa \
-I dedup_sample1.bam \
-L targets.interval_list \ #使用exome数据时,可用该参数指定文件(exome targets);也可以直接指定某染色体,如chr22
--genotyping_mode DISCOVERY \ #默认参数;GENOTYPE_GIVEN_ALLELES,仅使用给定文件中的allele
-stand_call_conf 30 \ #minimum confidence threshold (phred-scaled) at which the program should emit variant sites as called; 低于该值则标记为“LowQual"
-ERC GVCF \
-o sample1.raw_variants.g.vcf