ManNAz（四酰化 N-叠氮基乙酰基甘露糖胺）
2022-11-03 10:28 作者:BIOFOUNT范德生物 | 我要投稿
ManNAz（四酰化 N-叠氮基乙酰基甘露糖胺）是一种叠氮化物标记的糖，提供了一种高特异性方法，ManNAz被细胞转化为叠氮唾液酸衍生物，用于细胞表面蛋白的N-连接糖基化。主要用于通过体内代谢标记和化学选择性连接研究糖蛋白。
溶解度：Soluble in DMSO
性状：Solid powder
储藏条件：Dry, dark and store at 0-4℃ for short term (days to weeks) or -20℃ for long term (Store correctly 2-3years).
案例：
Detection of metabolic labeling with azido-sugars in different cell types using azide-reactive DyLight Dyes
A549, U2OS and HK-2 cells were incubated with 40µM azido-acetylmannosamine in cell culture media for 72 hours and then incubated with 100µM of DyLight 550-Phosphine (yellow). The cells were washed, fixed with 4% paraformaldehyde and counterstained with Hoechst 33342 (blue).
