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WSP-5(H2S Probe,硫化氢探针)

2022-11-16 10:04 作者:BIOFOUNT范德生物  | 我要投稿

WSP-5(H2S Probe)是一种新型 硫化氢荧光探针,WSP-5 用于硫化氢 (H2S) 的开启荧光探针,1 在与 H2S 反应时,WSP-5 会释放出荧光团,该荧光团的激发/发射最大值分别为 502/525 nm。 与 WSP-1 相比,WSP-5 具有更快的开启速率和对 H2S 更灵敏。

溶解度Solubility25 mg/ml in DMSO, 30 mg/ml in DMF

性状Solid,power

储藏条件 Storage conditions储存条件:-20° C,避光防潮密闭干燥。 运输条件:2~8℃运输

案例:

Fluorescence images of H 2 S in HeLa cells using WSP4 and WSP5. (a-f) Cells on 24-well plate were incubated with WSP4 (30 mm) for 30 min, then washed and subjected to different treatments: (a,b) Control (no NaHS was added); (c,d) treated with 30 mm NaHS; (e,f) treated with 60 mm NaHS. (g-l) Cells on 24-well plate were incubated with WSP5 (50 mm) for 30 min, then washed and subjected to different treatments: (g,h) Control (no NaHS was added); (i,j) treated with 50 mm NaHS; (k,l) treated with 100 mm NaHS. Scale bar: 100 nm.

文献:

1. Peng, B., Chen, W., Liu, C., et al. Fluorescent probes based on nucleophilic substitution-cyclization for hydrogen sulfide detection and bioimaging. Chemistry 20(4), 1010-1016 (2014).

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