CYTO-ID自噬检测试剂盒相关参数说明和研究方案
CYTO-ID® Autophagy Detection Kit 2.0 measures autophagic vacuoles and monitors autophagic flux in lysosomally inhibited live cells using a novel dye that selectively labels accumulated autophagic vacuoles. The dye has been optimized through the identification of titratable functional moieties that allow for minimal staining of lysosomes while exhibiting bright fluorescence upon incorporation into pre-autophagosomes, autophagosomes, and autolysosomes (autophagolysosomes). The assay offers a rapid and quantitative approach to monitoring autophagy in live cells without the need for cell transfection.
1、No transfection assay eliminates need for transfection efficiency validation
2、Brighter, more photostable dye specifically stains autophagic vesicles
3、Negligible staining of lysosomes reduces background seen with other dyes
4、Rapidly quantifies autophagy in native heterogeneous cell populations
5、Facilitates high-throughput screening of activators and inhibitors of autophagy
Applications:Flow Cytometry, Fluorescence microscopy, Fluorescent detection, HTS
Application Notes: The CYTO-ID® Autophagy detection kit 2.0 provides a rapid, specific, and quantitative approach for monitoring autophagy in live cells by fluorescence microscopy, flow cytometry, and microplate reader.
Quantity: For -0200 size:
200 flow cytometry assays, 250 microscopy assays or 3 x 96-well microplate assays
For -0050 size:
50 flow cytometry assays, 60 microscopy assays or 1 x 96-well microplate assays.
Use/Stability:With proper storage, the kit components are stable for one year from date of receipt.
Handling: Protect from light. Avoid freeze/thaw cycles.
Shipping: Shipped on Blue Ice
Short Term Storage:-20°C
Long Term Storage:-80°C
Contents: CYTO-ID® Green Detection Reagent 2
Hoechst 33342 Nuclear Stain
Autophagy Inducer (Rapamycin)
Chloroquine Control
10X Assay Buffer
艾美捷 Enzo CYTO-ID ®自噬检测试剂盒特点：
1.没有转染试验消除了转染效率验证的需要
2.更亮、更耐光的染料可特异性染色自噬囊泡
3.溶酶体的可忽略不计的染色降低了其他染料的背景
4.快速量化天然异质细胞群中的自噬
5.促进自噬激活剂和抑制剂的高通量筛选
艾美捷 Enzo CYTO-ID ®自噬检测试剂盒化学性质：
应用：流式细胞术、荧光显微镜、荧光检测、HTS
质量控制：显微镜检查：当 CYTO-ID® Green 自噬检测试剂 2 掺入细胞中时，通常会在细胞核周区域的球形空泡中、在整个细胞质中分布的病灶中或在这两个位置中观察到这种荧光探针的积累，具体取决于根据正在研究的细胞类型。与按照用户手册所述制备的自噬样品相比，未诱导的细胞显示出非常低的背景染色。
数量：对于 -0200 尺寸：
200 次流式细胞仪检测、250 次显微镜检测或 3 x 96 孔微孔板检测。
-0050 尺寸：
50 次流式细胞仪检测、60 次显微镜检测或 1 x 96 孔微孔板检测。
使用/稳定性：通过适当的存储，试剂盒组件自收到之日起可稳定使用一年。
处理：避光。避免冷冻/解冻循环。
在蓝冰上运送
短期储存：-20°C
长期储存：-80°C
艾美捷 Enzo CYTO-ID ®自噬检测试剂盒相关研究：
NUCLEAR-ID ® Red DNA 染料 ENZ-52406
PROTEOSTAT ® Aggregsome 检测试剂盒 ENZ-51035
MITO-ID ® Red 检测试剂盒 ENZ-51007
红色过氧化氢检测试剂盒 ENZ-51004
NUCLEAR-ID ®绿色染色质凝聚检测试剂盒 ENZ-51021-K200
NUCLEAR-ID ®蓝色 DNA 染料 ENZ-CHM103-0200
NUCLEAR-ID ® Blue/Red 细胞活力试剂 ENZ-53005-C100
