【标题速读】【nbt】【2023年】【1-4月】
声明：本专栏主要对生命科学领域的一些期刊文章标题进行翻译，所有内容均由本人手工整理翻译。由于本人专业为生物分析相关，其他领域如果出现翻译错误请谅解。
1.SARS-CoV-2–human protein–protein interactome. Rendering of complexes between SARS-CoV-2 and human proteins, with interface mutations highlighted. Zhou et al. and Kim et al. generate protein–protein interaction maps providing insights for potential therapeutic targets.
SARS-CoV-2-人类蛋白质-蛋白质相互作用组。 SARS-CoV-2 和人类蛋白质之间的复合物渲染，突出显示了界面突变。周和金等人生成蛋白质-蛋白质相互作用图，为潜在的治疗靶点提供见解。
2.Prediction of peptide mass spectral libraries with machine learning.
通过机器学习预测肽质谱库。
3.Automated reconstruction of whole-embryo cell lineages by learning from sparse annotations.
通过学习稀疏注释自动重建全胚胎细胞谱系。
4.Increasing the throughput of sensitive proteomics by plexDIA.
通过 plexDIA 提高敏感蛋白质组学的通量。
5.Uncovering the mode of action of engineered T cells in patient cancer organoids.
揭示工程 T 细胞在患者癌症类器官中的作用模式。
6.Bioengineered corneal tissue for minimally invasive vision restoration in advanced keratoconus in two clinical cohorts.
生物工程角膜组织用于两个临床队列中晚期圆锥角膜的微创视力恢复。
7.Removing unwanted variation from large-scale RNA sequencing data with PRPS.
使用 PRPS 消除大规模 RNA 测序数据中不需要的变异。
8.High-throughput continuous evolution of compact Cas9 variants targeting single-nucleotide-pyrimidine PAMs.
针对单核苷酸嘧啶 PAM 的紧凑型 Cas9 变体的高通量连续进化。
9.High-fidelity Cas13 variants for targeted RNA degradation with minimal collateral effects.
高保真 Cas13 变体用于靶向 RNA 降解，且附带影响最小。
10.The role of transposon inverted repeats in balancing drought tolerance and yield-related traits in maize.
转座子反向重复序列在平衡玉米耐旱性和产量相关性状中的作用。
11.A comprehensive SARS-CoV-2–human protein–protein interactome reveals COVID-19 pathobiology and potential host therapeutic targets.
全面的 SARS-CoV-2-人类蛋白质-蛋白质相互作用组揭示了 COVID-19 病理学和潜在的宿主治疗靶点。
12.A proteome-scale map of the SARS-CoV-2–human contactome.
SARS-CoV-2-人类接触组的蛋白质组规模图。
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1.Synthetic circRNA optimization. Circular RNA expression is affected by factors such as the placement of specific untranslated region and internal ribosome entry site features. Chen et. al. engineer and optimize synthetic circRNAs to allow robust translation of therapeutic proteins such as human erythropoietin.
合成 circRNA 优化。环状RNA的表达受特定非翻译区的位置和内部核糖体进入位点特征等因素的影响。陈等人设计和优化合成的 circRNA，以实现治疗性蛋白质（例如人类血清蛋白）的稳健翻译。
2.A competitive precision CRISPR method to identify the fitness effects of transcription factor binding sites.
一种有竞争力的精确 CRISPR 方法，用于识别转录因子结合位点的适应性效应。
3.Mostly natural sequencing-by-synthesis for scRNA-seq using Ultima sequencing.
主要是使用 Ultima 测序对 scRNA-seq 进行自然合成测序。
4.Multiplexed, single-molecule, epigenetic analysis of plasma-isolated nucleosomes for cancer diagnostics.
用于癌症诊断的血浆分离核小体的多重、单分子表观遗传学分析。
5.Scalable in situ single-cell profiling by electrophoretic capture of mRNA using EEL FISH.
使用 EEL FISH 通过电泳捕获 mRNA 进行可扩展的原位单细胞分析。
6.DeepConsensus improves the accuracy of sequences with a gap-aware sequence transformer.
DeepConsensus 通过间隙感知序列转换器提高了序列的准确性。
7.Post-translational modifications reshape the antigenic landscape of the MHC I immunopeptidome in tumors.
翻译后修饰重塑了肿瘤中 MHC I 免疫肽组的抗原景观。
8.Human ureteric bud organoids recapitulate branching morphogenesis and differentiate into functional collecting duct cell types.
人输尿管芽类器官再现了分支形态发生并分化为功能性集合管细胞类型。
9.Engineering circular RNA for enhanced protein production.
工程化环状 RNA 以增强蛋白质产量。
10.Modular cytokine receptor-targeting chimeras for targeted degradation of cell surface and extracellular proteins.
模块化细胞因子受体靶向嵌合体，用于细胞表面和细胞外蛋白的靶向降解。
11.Real-time denoising enables high-sensitivity fluorescence time-lapse imaging beyond the shot-noise limit.
实时降噪可实现超出散粒噪声限制的高灵敏度荧光延时成像。
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1.Super-resolution imaging. Artistic rendering of a system for noise reduction in super-resolution images. Qiao et al. incorporate prior knowledge of illumination patterns into a deep learning algorithm and apply this method to sustained live cell imaging.
超分辨率成像。超分辨率图像降噪系统的艺术渲染。乔等人将照明模式的先验知识融入深度学习算法中，并将该方法应用于持续的实时活细胞成像。
2.Modeling intercellular communication in tissues using spatial graphs of cells.
使用细胞空间图对组织中的细胞间通讯进行建模。
3.Engineered CRISPR prime editors with compact, untethered reverse transcriptases.
具有紧凑、不受束缚的逆转录酶的工程化 CRISPR prime 编辑器。
4.Quantitative sequencing using BID-seq uncovers abundant pseudouridines in mammalian mRNA at base resolution.
使用 BID-seq 的定量测序以碱基分辨率揭示了哺乳动物 mRNA 中丰富的假尿苷。
5.Absolute quantification of single-base m6A methylation in the mammalian transcriptome using GLORI.
使用 GLORI 对哺乳动物转录组中单碱基 m6A 甲基化进行绝对定量。
6.Rationalized deep learning super-resolution microscopy for sustained live imaging of rapid subcellular processes.
合理化深度学习超分辨率显微镜，用于快速亚细胞过程的持续实时成像。
7.Precision mitochondrial DNA editing with high-fidelity DddA-derived base editors.
使用高保真 DddA 衍生碱基编辑器进行精确线粒体 DNA 编辑。
8.Multi-omic single-cell velocity models epigenome–transcriptome interactions and improves cell fate prediction.
多组学单细胞速度模拟表观基因组-转录组相互作用并改善细胞命运预测。
9.Discovery of drug–omics associations in type 2 diabetes with generative deep-learning models.
通过生成深度学习模型发现 2 型糖尿病的药物组学关联。
10.Precise DNA cleavage using CRISPR-SpRYgests.
使用 CRISPR-SpRYgests 精确切割 DNA。
11.Haplotype-aware analysis of somatic copy number variations from single-cell transcriptomes.
单细胞转录组体细胞拷贝数变异的单倍型感知分析。
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1.Fluorescence microscopy. Artistic rendering of nucleosomes labeled with a reversibly switchable green fluorescent protein and portrayed as spinning tops on a plate. Volpato et al. developed a method that extends fluorescence anisotropy measurements to large protein complexes.
荧光显微镜。用可逆可切换绿色荧光蛋白标记的核小体的艺术渲染，并被描绘成板上的旋转陀螺。沃尔帕托等人。开发了一种将荧光各向异性测量扩展到大型蛋白质复合物的方法。
2.Modular, programmable RNA sensing using ADAR editing in living cells.
在活细胞中使用 ADAR 编辑的模块化、可编程 RNA 传感。
3.Systematic discovery of recombinases for efficient integration of large DNA sequences into the human genome.
系统地发现重组酶，用于将大 DNA 序列有效整合到人类基因组中。
4.Drag-and-drop genome insertion of large sequences without double-strand DNA cleavage using CRISPR-directed integrases.
使用 CRISPR 引导的整合酶拖放大序列的基因组插入，无需双链 DNA 切割。
5.Spatial mapping of the total transcriptome by in situ polyadenylation.
通过原位聚腺苷酸化对总转录组进行空间定位。
6.High-yield genome engineering in primary cells using a hybrid ssDNA repair template and small-molecule cocktails.
使用混合 ssDNA 修复模板和小分子混合物在原代细胞中进行高产基因组工程。
7.A split, conditionally active mimetic of IL-2 reduces the toxicity of systemic cytokine therapy.
IL-2 的分裂、条件活性模拟物可降低全身细胞因子治疗的毒性。
8.Targeting MYC with modular synthetic transcriptional repressors derived from bHLH DNA-binding domains.
使用源自 bHLH DNA 结合域的模块化合成转录阻遏物靶向 MYC。
9.Extending fluorescence anisotropy to large complexes using reversibly switchable proteins.
使用可逆可切换蛋白质将荧光各向异性扩展到大型复合物。
10.An engineered T7 RNA polymerase that produces mRNA free of immunostimulatory byproducts.
一种工程 T7 RNA 聚合酶，可产生不含免疫刺激副产物的 mRNA。
11.MINSTED nanoscopy enters the Ångström localization range.
MINSTED 纳米显微镜进入 Ångström 定位范围。
