【热门产品推介】InVivoMAb Anti-BRSV G/Major surface glycoprotein G Antib
货号：VVV25001
产品链接：http://www.antibodysystem.com/product/14666.html
寄主物种：Mouse
物种反应性：Bovine respiratory syncytial virus (strain 220-60) (BRS)
形式：Liquid
存储缓冲区：0.01M PBS, pH 7.4.
浓度：1 mg/ml
纯度：>95% by SDS-PAGE.
克隆性：Monoclonal
种型：IgG2a
应用：Neutralization
储存：Use a manual defrost freezer and avoid repeated freeze-thaw cycles.Store at +4°C short term (1-2 weeks).Store at -20 °C 12 months. Store at -80°C long term.
参考文献：
Serological and genetic characterisation of bovine respiratory syncytial virus (BRSV) indicates that Danish isolates belong to the intermediate subgroup: no evidence of a selective effect on the variability of G protein nucleotide sequence by prior cell culture adaption and passages in cell culture or calves. PMID: 9791873
Recognition of bovine respiratory syncytial virus proteins by bovine CD8+ T lymphocytes. PMID: 12562331
Antigenic diversity of respiratory syncytial viruses and its implication for immunoprophylaxis in ruminants. PMID: 8116189
An intranasal recombinant NDV-BRSV Fopt vaccine is safe and reduces lesion severity in a colostrum-deprived calf model of RSV infection. PMID: 36581658
Bovine respiratory syncytial virus infection. PMID: 17257568
Occurrence and phylogenetic analysis of bovine respiratory syncytial virus in outbreaks of respiratory disease in Norway. PMID: 24423030
HRSV主要导致急性下呼吸道感染，常见症状为咳嗽、咯痰、喘憋和发热及呼吸音异常，严重者会出现哮喘综合征、呼吸道阻塞、肺不张甚至窒息死亡，个别患儿会出现皮疹、弥漫性红斑等并发症。婴幼儿中死亡率较高的主要是早产儿、先天性心脏病等患者，老年人中为慢性肺疾病以及免疫缺陷患者。HRSV主要通过飞沫传播或经污染的手和物体表面传播，经口、鼻感染，潜伏期为3～7 d。
HRSV物理稳定性极差，在体外组织细胞中培养较难，新鲜标本、床边接种可以提高分离率。咽拭子、痰液等呼吸道标本均可用于HRSV培养分离，避免反复冻融防止降解。常用细胞系为喉癌细胞（Hep-2）、人Ⅱ型肺泡上皮细胞（A549）、人宫颈癌细胞系（HeLa）细胞。培养条件33～35 ℃，静止或旋转培养均可。因野生型强毒株在体外细胞组织中容易形成融合病变，因此通常使用噬斑法和微量细胞病变法对病毒进行滴定。病毒分离培养时，在病毒载量较低时很难引起细胞病变，第一代细胞病变往往不典型，容易造成假阴性的误判；盲传后在5 d内可出现细胞融合等典型病变，但细胞分离培养阳性具有较强的可信度。
