Product name: GAPDH Mouse mAb

Catalog No.: RAB0101

Storage Buffer & Condition: PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol.

Store at -20°C. Do not aliquot the antibody.

Recommended dilutions:WB: 1:2,000-10,000    IHC: 1:200-500    IF:1:100-500

Optimal dilutions should be determined by the end user.

Specificity: Antibody can detects endogenous GAPDH protein.

Background:  Glyceraldehyde 3 phosphate dehydrogenase (GAPDH) is well known as one of the key enzymes involved in glycolysis. GAPDH is constitutively expressed in almost all tissues at high levels, therefore antibodies against GAPDH are useful as loading controls for Western Blotting. Some physiological factors, such as hypoxia and diabetes, increase GAPDH expression in certain cell type.

使用本产品文献：

Toll‐like receptor 2 mediates the degeneration of cartilage in experimental inflammatory TMJOA

X Liu, Y Feng, W Liu, H Li, Z Hu, S Hu, J Ke… - Oral …, 2020 - Wiley Online Library

… anti-GAPDH (1:5000, RAB0101, FriendBio, Wuhan, China), rabbit anti-TLR2 (1:1000), rabbit …
Acknowledgement This work was supported by National Natural Science Foundation of China
(Grant No. 81771100) … production by Saos-2 cells. Life sciences 78: 2697-706 …