【MIT细胞生物学】复习大纲 07-09
学习顺序与主题 LEARNING SEQUENCES AND TOPICS
07 核孔
08 细胞核转运的分子机制
09 核运输的检测
9.3 活化T细胞核内因子-钙调神经磷酸酶途径 (NFAT-CaN途径)
目标 GOALS
1. Understand how the structure of the nuclear pore complex influences transport in and out of the nucleus.
了解核孔复合体的结构如何影响细胞核跨膜运输。
2. Detail the interactions between molecular machinery that facilitates nuclear transport.
详细说明促进细胞核跨膜运输的分子相互作用机制。
你应该能做到 You should be able to
Contrast movement of proteins in nuclear transport to those in membrane trafficking.
对比核转运和膜转运中的蛋白运动。
Explain how the structures of the nuclear envelope and nuclear pore complex affect nuclear transport.
解释核被膜与核孔复合体的结构如何影响核运输。
Determine how molecules will be transported in and out of the nucleus based on their cellular function.
根据细胞功能，确定分子如何进出细胞核。
Experimentally determine nuclear localization and export sequences.
实验确定核定位和输出序列。
Explain the function of nuclear localization and export sequences.
解释核定位和输出序列的功能。
Identify the required proteins for nuclear transport.
确定细胞核运输所需的蛋白质。
Summarize the steps for nuclear import and export.
总结蛋白质进出细胞核的步骤。
Describe how the cell establishes and maintains Ran asymmetry.
描述细胞如何建立和维持Ran不对称性。
Propose experiments to characterize the proteins involved in nuclear transport.
设计实验来表征参与核运输的蛋白质。
Explain how Förster (or Fluorescence) Resonance Energy Transfer (FRET) can be used to study the location and interactions of proteins.
解释荧光共振能量转移技术（FRET）如何用于研究蛋白质的位置和相互作用。
Interpret a FRET experiment.
解释FRET实验。
Predict how specific post-translational modifications regulate nuclear transport.
预测特定的翻译后修饰如何调节核运输。
Explain why maintaining high specificity and rapid flux through the nuclear pore is especially perplexing.
解释为什么通过核孔保持高特异性和快速通量特别令人困惑。
