【液相应用】针运行出现错误Needle/Seal move limit exceeded

菜鸟博士

运行期间出现错误：针/密封移动超出限制 (5.0) Needle/Seal move limit exceeded (5.0) - 中文版

故障描述

运行期间或 SM-FTN 灌注期间出现 Needle/Seal move limit exceeded (5.0)（针/密封移动超出限制 (5.0)）错误信息

“定义针密封”诊断测试通过，未出现问题或错误

环境

ACQUITY UPLC H-Class

ACQUITY UPLC I-Class

SM-FTN

原因

密封常数过低 (3 N/mm)，需要重置称重传感器线缆。

安装 30 μL 针时也会出现问题，因为针密封（使用该针头）定义会给出较低的密封常数值。针密封定义应使用缺省的 (15 μL) 针进行，然后可以更换为 30 μL 针，无需进一步定义。

解决方法

重新启动 SM-FTN。此步骤在更换针后至关重要（在 15 μL 和 30 μL 针之间更换）。

运行“定义针密封件”诊断测试，确保密封常数接近 8-11 N/mm。

SYMPTOMS

Error message "Needle/Seal move limit exceeded (5.0)" appears during runs or during priming of SM-FTN

"Characterize Needle Seal" diagnostic passes without issue or error

ENVIRONMENT

ACQUITY UPLC H-Class

ACQUITY UPLC I-Class

SM-FTN

CAUSE

The seal constant is too low (3 N/mm). The load cell cable needs to be reset.

Needle carriage wear issue , loose belt from the needle carriage can cause this error.

Problem will occur also when 30 uL needle is fitted as the characterization of the needle seal (with that needle) will give low value of seal constant. The characterization of the needle seal shall be performed with default (15 uL) needle and then it can be replaced with 30 uL needle without further characterizations.

FIX or WORKAROUND

Power-cycle the SM-FTN. This step is crucial after needle replacement (changing between 15uL and 30uL needles).

Run the "characterize needle seal" diagnostic and ensure the seal constant is closer to 8-11 N/mm.