中英文丨预激活APC-Cy7串联说明书

Allophycocyanin (APC) is a phycobiliprotein isolated from Spirulina sp., a blue-green alga. Like other phycobiliproteins, APC is fluorescent, with an extremely high absorptivity and a high quantum efficiency. It is a protein which can be easily linked to antibodies and other proteins by conventional protein cross-linking techniques without altering its spectral characteristics. APC-Cy7 is a popular color used in flow cytometry. Its primary absorption peak is at 651 nm with emission peak at~780 nm.

 

AAT Bioquest offers this preactivated APC-Cy7 to facilitate the APC-Cy7 tandem conjugations to antibodies and other proteins such as streptavidin and other secondary reagents. Our preactivated APC-Cy7 tandem is ready to conjugate, giving much higher yield than the conventionally tedious SMCC-based conjugation chemistry. In addition, our preactivated APC-Cy7 tandem is conjugated to a protein via its amino group that is abundant in proteins while SMCC chemistry targets the thiol group that has to be regenerated by the reduction of antibodies.

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别藻蓝蛋白 (APC) 是一种从蓝绿藻螺旋藻中分离出来的藻胆蛋白。与其他藻胆蛋白一样，APC 是荧光的，具有J高的吸收率和高量子效率。它是一种可以通过常规蛋白质交联技术轻松与抗体和其他蛋白质连接而不改变其光谱特性的蛋白质。APC-Cy7 是流式细胞术中常用的颜色。其主要吸收峰在 651 nm，发射峰在 ~780 nm。

 

艾美捷预激活APC-Cy7串联 以促进 APC-Cy7 与抗体和其他蛋白质（如链霉亲和素和其他二级试剂）的串联偶联。该预激活APC-Cy7串联已准备好进行偶联，与传统繁琐的基于 SMCC 的偶联化学相比，其产量要高得多。

 

艾美捷预激活APC-Cy7串联普通储备溶液制备：

将特定质量的ReadiUse™ 预活化 APC-Cy7 Tandem复溶至给定浓度所需的适当溶剂体积。请注意，体积仅用于制备储备溶液。

 

艾美捷预激活APC-Cy7串联组件：

A：ReadiUse™ 预激活 APC-Cy7 串联 1 瓶（1 毫克）

B：Buccutite™ MTA 1 瓶 (100 µg)

C：旋转脱盐柱 不包含

 

光谱特性：

消光系数（cm -1 M -1） 700000

激发（纳米） 651

发射（纳米） 779

 

储存、安全和处理：

H-短语 H303、H313、H333

危险符号 XN

有可能的使用 仅供研究使用 (RUO)

R-短语 R20、R21、R22

UNSPSC 12171501