中英文丨人干扰素Y ELISA开发试剂盒说明书

Analyte Information

IFN-γ

Interferon-γ (IFN-γ, also known as IFN-gamma, IFN-g, IFNg and Interferon-gamma) is produced mainly by activated T cells and NK cells. It is a proinflammatory cytokine that activates macrophages and endothelial cells, but it also regulates immune responses by effecting APC and T and B cells. Production of IFN-γ by helper T cells as well as cytotoxic T cells is a hallmark of the TH1-type phenotype, thus, high-level production of IFN-γ is typically associated with effective host defense against intracellular pathogens.

ELISABASIC kit for quantification of IFN-γ in solution

The human IFN-γ ELISABASIC kit is ideal for users who want the flexibility of setting up their own ELISA assay. This product enables quantification of human IFN-γ in solution, e.g. in cell culture supernatant. The kit contains a matched pair of monoclonal capture and detection antibodies, Streptavidin-HRP, ELISA standard and standard reconstitution buffer. Plates and substrate are not included.

Human IFN-γ ELISABASIC kits are also available with Streptavidin-ALP.

Serum/Plasma samples

Analysis of serum/plasma samples with this kit requires the use of ELISA diluent for dilution of the samples, standard and detection antibody. The diluent prevents false positive read-outs which may be caused by interference of heterophilic antibodies found in serum and plasma. The ELISA diluent is available from Mabtech. See Related products.

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艾美捷人干扰素Y ELISA开发试剂盒，用于定量溶液中的 IFN-γ

人干扰素Y ELISA开发试剂盒非常适合希望灵活设置自己的 ELISA 检测的用户。该产品可以对溶液中的人 IFN-γ 进行定量，例如在细胞培养上清液中。该试剂盒包含一对匹配的单克隆捕获和检测抗体、Streptavidin-HRP、ELISA 标准和标准重组缓冲液。不包括板和基板。人干扰素Y ELISA开发试剂盒也可与 Streptavidin-ALP 一起使用。

血清/血浆样本

使用艾美捷人干扰素Y ELISA开发试剂盒分析血清/血浆样品，需要使用 ELISA 稀释剂来稀释样品、标准品和检测抗体。稀释剂可防止由于血清和血浆中发现的异嗜性抗体的干扰而导致的假阳性读数。ELISA稀释剂可从Mabtech获得。仅供研究使用 (RUO)。不用于诊断过程。

艾美捷人干扰素Y ELISA开发试剂盒标准曲线的绘制：

艾美捷人干扰素Y ELISA开发试剂盒内容：

捕获 mAb (1-D1K)

生物素化检测 mAb (7-B6-1)

链霉亲和素-HRP

重组人 IFN-γ ELISA 标准品

标准重组缓冲液

应用：酶联免疫吸附试验

反应性：人类

标准范围：4-400 皮克/毫升