解决方案丨艾美捷海肾荧光素酶测定试剂盒
海肾荧光素酶测定试剂盒2.0是Biotium的海肾荧光素酶测定试剂盒（目录号30004）的改进版本，具有简化的方案和改进的工作溶液稳定性。该试剂盒还具有Biotium的水溶性Aquaphile腔肠素。
艾美捷海肾荧光素酶测定试剂盒2.0,150次测定#BTM-30082-1组件：
5X 被动裂解缓冲液
海肾萤光素酶检测缓冲液 2.0
Aquaphile™ 腔肠素
海肾荧光素酶测定试剂盒相关特征：
高灵敏度和线性快速型 RLuc 测定
易于处理的水溶性基材
高性价比
海肾荧光素酶测定试剂盒属性：
化验类型/选项：终点测定，发光（闪光型）
检测方法/读数：光度计（带试剂注射器的单管或微量板读取器）
储存条件：在-60°C或更低温度下储存
Sea kidney Luciferase has been used as a reporter gene to study gene regulation and function in vitro and in vivo. It is usually used for multiple transcriptional reporter gene detection or as a standardized transfection control for firefly Luciferase detection. Renila Luciferase is a 36000 Dalton monomer protein that catalyzes the oxidation of coelenterazine by oxygen to produce light. The activity of this enzyme does not require post translational modification and can serve as a genetic reporter gene immediately after translation. Coelenterazine also emits light through enzyme independent oxidation, a process called self luminescence. Superoxide anions and peroxynitrite in cells and tissues enhance self luminescence. This test kit uses a special buffer formula, which aims to conduct reliable linear measurement of Luciferase activity in the sea and kidney with the minimum self luminous background and excellent sensitivity.
Note: This reagent kit is a flash type luminescence detection that requires immediate measurement of luminescence after adding reagents to your sample. It requires a single tube luminescent meter or a luminescent enzyme-linked immunosorbent assay instrument with a reagent syringe.
Easy to use for consistent results
This kit is a new improved substitute for Haishen Luciferase Test Kit (Catalog No. 30004). In addition to providing a simplified experimental scheme, Renilla Luciferase Test Kit 2.0 also has Biotium Aquaphile ™ Coelenterazine, a water-soluble substrate, is different from coelenterazine's methanol solution. It can be stored at - 20 ° C with the minimum evaporation.
The measurement quantity is based on a 96 well measurement format. This kit contains sufficient passive lysis buffer to perform the specified number of measurements within the plate size range of 96 to 24 wells.
