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17β-雌二醇ELISA试剂盒,安全无放射性酶法测定

2023-01-10 16:22 作者:艾美捷  | 我要投稿

The 17β-Estradiol EIA kit is a colorimetric competitive enzyme immunoassay kit with results in < 3 hours. Absorbance is read at 405 nm. This kit offers specific and save non-radioactive quantification of estradiol with ready-to-use liquid color-coded reagents to reduce error.

Specific with low cross-reactivity to related steroids

Safe and non-radioactive enzymatic assay

Simple and color-coded reagents reduces error

Reliable and reproducible results day-after-day and lot-after-lot

Fully quantitative results that surpass semi-quantitative Western blot analysis

Alternative Name: Estrogen

Sensitivity: 28.5 pg/ml (range 29.3 - 30,000 pg/ml)

Assay Time: <3 hours

Applications: ELISA, Colorimetric detection

Application Notes: For the quantitative determination of 17β-Estradiol in culture supernatants and saliva from any species. For serum and plasma use ADI-900-174.

Wavelength: 405 nm

Species reactivity: Species independent

Shipping: Blue Ice Not Frozen

Long Term Storage: +4°C

Contents: GxR IgG Microtiter plate, Conjugate, Antibody, Assay buffer 3, Wash buffer concentrate, Standard, pNpp Substrate, Stop solution

Scientific Background: Biologically active estrogen, 17β-estradiol, is an important sex hormone, but also makes an important contribution to the high concentrations of estrogens, which are present in malignant breast tissues.

 

17β-雌二醇ELISA试剂盒是一种比色竞争酶免疫测定试剂盒,结果在3小时以内。在405 nm处读取吸光度。该试剂盒使用现成的液体色码试剂提供雌二醇的特异性和节省的非放射性定量,以减少误差。

 

17β-雌二醇ELISA试剂盒艾美捷特点:

1.对相关类固醇具有低交叉反应性的特异性

2.安全无放射性酶法测定

3.简单且颜色编码的试剂减少了错误

4.日复一日、一批又一批可靠且可重复的结果

5.超过半定量蛋白质印迹分析的全定量结果

 

17β-雌二醇ELISA试剂盒化学性质:

别名:雌激素

灵敏度:28.5 pg/ml(范围29.3-30000 pg/ml)

化验时间:<3小时

应用:ELISA,比色检测

应用说明:用于定量测定任何物种的培养上清液和唾液中的17β-雌二醇。血清和血浆使用ADI-900-174。

波长:405 nm

物种反应性:物种无关

运输:蓝冰未冻结

长期储存:+4°C

内容:GxR IgG微量滴定板、缀合物、抗体、测定缓冲液3、洗涤缓冲液浓缩液、标准品、pNpp底物、停止溶液

 

17β-雌二醇ELISA试剂盒文献参考:

Organochlorine pesticide dieldrin upregulate proximal promoter (PII) driven CYP19A1 gene expression and increases estrogen production in granulosa cells: D. Sharma, et al.; Reprod. Toxicol. 106, 103 (2021), Abstract;

Encapsulation of mesenchymal stem cells in 3D ovarian cell constructs promotes stable and long-term hormone secretion with improved physiological outcomes in a syngeneic rat model: S. Sittadjody, et al.; Ann. Biomed. Eng. 48, 1058 (2020), Application(s): ELISA using culture supernatants, Abstract; Full Text

A high level of TGF-B1 promotes endometriosis development via cell migration, adhesiveness, colonization, and invasiveness: U.K. Soni, et al.; Biol. Reprod. 100, 917 (2019), Abstract;

CB1 agonism prolongs therapeutic window for hormone replacement in ovariectomized mice: K. Zhang, et al.; J. Clin. Invest. 130, 2333 (2019), Abstract;


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