Mag-Fura-2,细胞内镁荧光探针

Mag-fura-2 四钾盐是一种细胞内镁离子特异性荧光探针，属于紫外光激发的比率式探针。水溶性的盐形式适用于通过显微注射、从膜片移液管注入。

Mag-fura-2 四钾盐是一种细胞内镁离子特异性荧光探针，属于紫外光激发的比率式探针。水溶性的盐形式适用于通过显微注射、从膜片移液管注入。

溶解度：Solubility Soluble in water

性状：Solid power

储藏条件：torage at -4℃ (1-2weeks), longer storage period at -20℃ (1-2years)

案例：

The effects of Mrs2 KD on the intracellular Mg2+ homeostasis.: The images of HeLa cells simultaneously loaded with both Mag-Fura-2 (a) and MitoFluor Red (b). (c–f) The enlarged view of the boxed area in panel a and b. (c,d) The localization of Mag-Fura-2 in the same cell shown in the panel a and b before (c) and after (d) cell membrane permeabilization by digitonin. (e) Localization of MitoFluor Red after cell membrane permeabilization by digitonin. (f) The merged image were shown. (g) The mitochondrial Mg2+ level in control and Mrs2 KD HeLa cells. Comparing the ratio of Mag-Fura-2 fluorescence excited at 340 nm to at 380 nm, indicating mitochondrial Mg2+ level, revealed a decrease in Mrs2 KD cells (n = 148 for the control and n = 105 for Mrs2 KD cells from 5 experiments). (h–j) The images of HeLa cells normally loaded with Mag-Fura-2. (h) Fluorescence image of Mrs2 KD cells labeled with emGFP. (i) The merged fluorescence images of control and emGFP- labeled (red) Mrs2 KD cells loaded with Mag-Fura-2 (green). (j) The fluorescence emission ratio image at excitation of 340 and 380 nm of HeLa cells loaded with Mag-Fura-2. Pseudo color showed the emission ratio of Mag-Fura-2 signals excited at 340 nm to 380 nm. (k) The cytosolic Mg2+ level in control and Mrs2 KD cells. In Mrs2 KD cells, cytosolic Mg2+ level is higher than that in control cells (n = 304 for the control and n = 384 Mrs2 KD cells from 5 experiments).