中英文说明书丨艾美捷FD Apop TUNEL凋亡检测试剂盒

FD Apop™ Kit is designed for the microscopic detection of cells undergoing apoptosis based on the principle of in situ DNA nick-end labeling (TUNEL) technique¹. The assay uses terminal deoxynucleotidyl transferase to catalyze the incorporation of biotinylated deoxyuridines onto the free 3′-hydroxyl termini of DNA fragments, which are considered one of the most characteristic features of apoptosis2, 3. The integrated biotins are amplified and visualized with the avidin-biotin-complex (ABC) method4, enabling light microscopic identification.

The reagents and procedure of FD Apop™ Kit have been optimized to achieve a high degree of both specificity and sensitivity for detecting apoptotic cells with minimal background. This kit can be used with frozen and paraffin sections, as well as cultured cells. The procedure of the kit takes approximately 4 hours.

Kit contents:

Part I (Store at -20°C)

Digestive Enzyme                            2 ml x 4

Reaction Solution A                         2 ml x 2

Reaction Solution B                         60 µl

Reaction Solution C                         40 µl

Chromogen Solution                        20 ml

Part II (Store at 4°C)

Equilibration Buffer                          20 ml

Detection Reagent                           6 ml

10x Phosphate-Buffered Saline    250 ml x 2

艾美捷FD NeuroTechFD Apop TUNEL凋亡检测试剂盒（#PK101）设计用于基于原位DNA切口标记（TUNEL）技术原理的细胞凋亡的显微镜检测。该测定使用末端脱氧核苷酸转移酶催化生物素化脱氧尿苷掺入DNA片段的游离3′-羟基末端，这被认为是细胞凋亡极为典型的特征之一2, 3.整合的生物素通过亲和素-生物素复合物（ABC）方法进行扩增和可视化4，可实现光学显微镜识别。

FD NeuroTech FD Apop TUNEL凋亡检测试剂盒的试剂和程序已经过优化，可实现高度的特异性和灵敏度，以极小的背景检测凋亡细胞。该试剂盒可用于冷冻和石蜡切片以及培养细胞。该套件的过程大约需要 4 小时。

FD Apop TUNEL凋亡检测试剂盒文献引用：

1.Gavrieli Y, Sherman Y, and Ben-Sasson SA. (1992) Identification of programmed cell death in situ via specific labeling of nuclear DNA fragmentation. J. Cell Biol. 119:493-501.

2.Wyllie AH. (1980) Glucocorticoid-induced thymocyte apoptosis is associated with endogenous endonuclease activation. Nature. 284:555-6.

3.Arends MJ, Morris RG, and Wyllie AH. (1990) Apoptosis: the role of the endonuclease. Amer. J. Pathol. 136:593-608.

4.Hsu SM, Raine L, and Fanger H. (1981) Use of avidin-biotin-peroxidase complex (ABC) in immunoperoxidase techniques: a comparison between ABC and unlabeled antibody (PAP) procedures. J. Histochem. Cytochem. 29:577-80.