荧光素二-β-D-半乳糖苷 (FDG)

Fluorescein di-ß-D-galactopyranoside (荧光素二-β-D-半乳糖苷,FDG) 用于体内和体外 lacZ ß-半乳糖苷酶检测的超灵敏底物。fluorescein di-ß-D-galactopyranoside (FDG)广泛用于通过流式细胞术检测哺乳动物细胞和革兰氏阴性菌中的大肠杆菌 lacZ β-半乳糖苷酶。 进入活细胞。 与 lacZ酶活性 成比例的荧光信号。

溶解度：DMSO: soluble

性状：Solid

储藏条件：Storage conditionsstorage at -4℃ (1-2weeks), longer storage period at -20℃ (1-2years)

Sternomastoid muscle fibers of a living mouse. YOYO-1 and fluorescein di-beta-D-galactopyranoside (FDG).

Sternomastoid muscle fibers of a living mouse that have been transfected with YOYO-1 dye–stained DNA (red) containing the <i>lacZ</i> reporter gene and then stained with the beta-galactosidase substrate fluorescein di-beta-D-galactopyranoside (FDG, F1179). DNA stained with YOYO-1 (Y3601) prior to implantation could still be localized five days after application. Fluorescence signals were visualized <i>in situ</i> by epifluorescence microscopy with a low–light level SIT camera and a computer imaging system. Image contributed by Peter van Mier, Washington University School of Medicine.

文献：

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