Research Grade Anti-RSV F/Fusion glycoprotein F0 (ADI-14
呼吸道合胞病毒（Respiratory Syncytial Virus, RSV）作为引起急性下呼吸道感染（ALRTIs）的主要原因之一，每年导致全球约3.31亿人发生呼吸道感染。然而，就是这样一个常见且早已被发现的呼吸道病毒，其疫苗的研发却十分坎坷，以至于至今仍无一款成熟的RSV疫苗问世。
此前的数十年间，科学家们不曾停止对于RSV疫苗的研究与开发，但均以失败告终。究其原因，可能是因为RSV病毒结构的特殊性。病毒表面的融合蛋白（fusion protein , F）和粘附蛋白（attachment protein, G）因在病毒入侵过程中的关键作用成为病毒研究的理想靶点，而其中G蛋白变异较多相对不稳定，F蛋白也存在Pre到Post构象转变影响其稳定性。
货号：DVV02811
产品链接：http://www.antibodysystem.com/product/9005.html
寄主物种：Human
形式：Liquid
存储缓冲区：0.01M PBS, pH 7.4.
浓度：1 mg/ml
纯度：>95% by SDS-PAGE.
克隆性：Monoclonal
应用：Research Grade Biosimilar
储存：Use a manual defrost freezer and avoid repeated freeze-thaw cycles.Store at +4°C short term (1-2 weeks).Store at -20 °C 12 months. Store at -80°C long term.
联系方式：027-65279366
为了克服这一障碍，科学家们对RSV F蛋白的蛋白结构及其抗体进行了研究，发现该蛋白表面至少存在7个完全不同的抗原表位，并成功筛选到可以与不同表位结合的抗体，分别为φ site_D25/5C4/AM22，I site_131-2A/2F，II site_motavizumab/palivizumab，III site_MPE8，IV site_101F/mAb19，V site_hRSV90。其中识别IV表位的101F、识别II表位的palivizumab和识别I表位的131-2A抗体可以同时识别F蛋白的pre和post构象，识别φ表位的D25抗体只与Pre构象的F蛋白发生结合，识别III表位的MPE8抗体则偏好识别Pre构象，与Post构象具有较弱的结合活性。2010年，Kwakkenbos研究团队筛选获得单克隆抗体AM14，其识别的抗原表位位于F-pre蛋白近赤道区域，横跨两个单体，可特异性识别F-pre三聚体结构，且被证明与此前中和效果最佳的D25抗体具有相似的中和活性和更快的动力学性质。
参考文献：
Dissociation of the respiratory syncytial virus F protein-specific human IgG, IgA and IgM response. PMID: 33574352
Cleavage of the respiratory syncytial virus fusion protein is required for its surface expression: role of furin. PMID: 10930660
Prefusion F, Postfusion F, G Antibodies, and Disease Severity in Infants and Young Children With Acute Respiratory Syncytial Virus Infection. PMID: 29029312
Intracellular processing of the human respiratory syncytial virus fusion glycoprotein: amino acid substitutions affecting folding, transport and cleavage. PMID: 1375280
Fusion glycoprotein of human parainfluenza virus type 3: nucleotide sequence of the gene, direct identification of the cleavage-activation site, and comparison with other paramyxoviruses. PMID: 3012869
Membrane-associated respiratory syncytial virus F protein expressed from a human rhinovirus type 14 vector is immunogenic. PMID: 11277694
Alkaline phosphatase fusions to the respiratory syncytial virus F protein as an approach to analyze its membrane topology. PMID: 2558867
Clinical Potential of Prefusion RSV F-specific Antibodies. PMID: 29054341
A highly stable prefusion RSV F vaccine derived from structural analysis of the fusion mechanism. PMID: 26333350
Structure-based design of a fusion glycoprotein vaccine for respiratory syncytial virus. PMID: 24179220
