Acridine Orange 10-Nonyl Bromide,线粒体荧光探针

Acridine Orange 10-Nonyl Bromide,线粒体荧光探针（10-壬基溴代吖啶橙）是一种异染性染料，可用作活细胞中的线粒体荧光探针。 它已被用于通过流式细胞术分析线粒体、表征多药耐药性以及测量大鼠胸腺细胞凋亡过程中线粒体质量的变化。荧光 :λex 496 nm, λem 524 nm in CH3OH.

10-壬基溴代吖啶橙 Acridine Orange 10-Nonyl Bromide (75168-11-5) 应用：
Acridine Orange 10-nonyl bromide is a metachromatic dye useful as a mitochondrial probe in living cells. It has been used to analyze mitochondria by flow cytometry, characterize multidrug resistance, and measure changes in mitochondrial mass during apoptosis in rat thymocytes.
10-壬基吖啶橙(NAO)是吖啶橙的衍生物, 是一种用于完整细胞内线粒体特异性荧光标记物。与Rh123（Mb1811）相比，细胞中NAO的积聚与质子动力驱动关系不大，但是与线粒体相关膜蛋白或者脂类发生作用相关。NAO的摄入不依赖于线粒体跨膜电势差，可用于检测线粒体。NAO可与心磷脂等带负电荷磷脂特异性结合, 其与线粒体膜的相互作用不依赖于线粒体的膜电位; 分析细胞凋亡时, 可用罗丹明123来检测线粒体的膜电位, 而用NAO来检测线粒体结构的完整性。NAO和罗丹明123（Rh123）是研究线粒体功能一对好的染料。

案例：

A: Fluorescence microscopy of Acridine orange 10-nonyl bromide (NAO) stained mitochondria (pseudo coloured red) in spongy myocardium. The segmentation masks are shown as green lines around the mitochondria. B: Quantification of fluorescence intensity inside the segmentation masks showed that mitochondrial area within the cardiomyocytes of Atlantic salmon was significantly increased by the high-intensity training regime (approx. 16000 mitochondrial from sections of 5 and 6 hearts from control and high-intensity regimes, respectively). The number of mitochondria per cell though, was not significantly different as shown by that the ratio between mitochondrial DNA and genomic DNA (mtDNA/gDNA). *: Student’s t-test p<0.05, performed on metric and log2ER values for area and mtDNA/gDNA respectively. n?=?12/group.