ANS-Na,疏水牛血清白蛋白荧光探针

ANS-Na (8-苯胺基-1-萘磺酸钠) 是一种疏水荧光染料，ANS 用于研究蛋白折叠/去折叠途径的中间状态的重要染料。也可用于检测与蛋白聚集有关的构象变化。ANS在水溶液中是非荧光的，但与BSA（牛血清白蛋白）和变性蛋白结合后发出强烈荧光。它可用于检测血清白蛋白。ANS主要与暴露于溶剂的非极性（疏水性）位点相互作用。

溶解度Solubility溶于水

性状Solid，power

储藏条件 Storage conditions4℃条件下存储,-4℃存储更佳

PH 对ANS探针的影响：

(A) Effect of pH on the binding of 8-anilino-1-naphthalenesulfonic acid (ANS) with CdGSTM1. The binding of ANS (200 μM) with CdGSTM1 (4 μM) at pH 1.0-10.0 was monitored by excitation at 380 nm (bandwidth 2.5 nm) and emission spectra collected from 400-600 nm (bandwidth 5 nm) at 22?C. (B) Rayleigh light scattering measurements of CdGSTM1 (0.1 mg mL-1 ) were obtained at 500 nm wavelength at pH 1.0-10.0.