氨基酸检测报告（蛹虫草）

项目报告

1 样品信息Sample description

收到蛹虫草样品xx个，a和b

A total of xx Cordyceps militaris samples, where received by the customer.

2 材料与方法Materials and Methods

2.1 试剂Chemicals

所有的溶剂与试剂均为色谱级，脯氨酸，缬氨酸，蛋氨酸，异亮氨酸，亮氨酸，苯丙氨酸标准品购于Sigma-Aldrch

All chemicals and solvent were HPLC. Standard: proline, valine, methionine, isoleucine, leucine, phenylalanine and were purchased from Sigma-Aldrch.

2.2 样品处理Sample Preparation

1) 将样品摇匀，称取2.5g左右的样品；

2) 2.5g样品加入100 mL xxx，摇匀，于微波炉中处理3 min；

3) 过滤，滤液用0.22 µm滤膜过滤，进样5 µL。

2.3 HPLC分析HPLC Analysis

本实验所用的仪器为LC-20A型高效液相色谱仪，配备蒸发光散射检测器。

色谱柱为xxx C18 (150 mm*4.6mm, 5 μm)，流动相A为xxx，B为水，流速为0.8 mL/min，柱温为40℃。

3 结果Results

色谱图Chromatogram