中英文说明丨艾美捷高通量NGS标准化96孔板试剂盒

Norgen's NGS Normalization 96-Well Kit allows for high-throughput PCR amplicon clean-up and normalization of PCR product concentration (~5 ng/µL). The clean-up removes all PCR by-products including primers, dimers, enzymes and unincorporated nucleotides. Purification is based on 96-well column chromatography using Norgen’s proprietary resin as the separation matrix. The amount of Norgen’s matrix in each 96 well is optimized to have a limited binding capacity, thus each well can elute an equal concentration of PCR product. The process involves first adding 3 volumes of Buffer SK to the PCR product, and the mixture is then loaded onto the 96-Well Normalization Plate. The bound PCR amplicon is then washed with the provided Wash Solution A in order to remove any remaining impurities, and the purified PCR amplicon is eluted with Elution Buffer B. The purified and normalized PCR amplicon can then be used in NGS workflows and other sequencing applications.

 

For high-throughput NGS Library PCR amplicon clean-up and normalization of NGS library PCR product concentration

 

NGS Normalization 96-Well Kit：

√Removes primer dimers

√Simultaneously clean-up and normalize PCR products

√Fast (less than 20 minutes), high-throughput and easy processing using centrifugation

√Sufficient elution volume (100 µL) for repeat or future assays

√Non-magnetic bead purification

 

艾美捷高通量NGS标准化96孔板试剂盒#NGB-61900：

中文名称：NGS标准化96孔板试剂盒

英文名字：NGS Normalization 96-Well Kit

Norgen Biotek编号：NGB-61900

规格：2Plates

保存建议：溶液密封室温(15-25°C)保存，未开封至少保存1年。

 

NGS标准化96孔试剂盒组件包含：

组件 NGS Normalization

Buffer SK 30 mL

Wash Solution A 2x 38 mL

Elution Buffer B 30 mL

Adhesive Tape 1

96-Well Normalization Plate 2

96-Well Collection Plate 2

96-Well Elution Plate 2

Product Insert 1

NGS标准化96孔试剂盒（NGS Normalization）储存条件所有溶液均应密封并在室温下储存。 本试剂盒在装运日期后2年内保持稳定。

 

高通量NGS标准化96孔板试剂盒特色：

√去除引物二聚体

√同时净化和标准化PCR产物

√快速（不到 20 分钟）、高通量且易于离心处理

√足够的洗脱体积 （100 μL），用于重复或将来的分析

√非磁珠纯化