分子与细胞生物学 22 - L8e Molecular Biology Lab Techniques (3)

本期的内容是第八章：分子生物学实验室技术：杂交技术。如果有不太明白的或者有错误的地方随时来找UP主喔~ 文集本部分的参考文献Essential Cell Biology, 5th ed. Alberts, et al. 2019. 部分内容来自khanacademy与维基百科。

本期封面/头图: 参见本期笔记图片的第一页。

本期内容的笔记以文本+图片的形式呈现，共计2页笔记图片。

8e Genetic Libraries

    A library is a collection of DNA fragments that is stored and propagated in a population of micro-organisms through the process of molecular cloning. Genetic libraries have a collection of different DNAs, each housed in a different bacteria.

Genomic DNA library

    A genomic library is a collection of the total genomic DNA from a single organism. Genomic library contains clones of all the sequences from the genome in the particular organism.

    Make a genomic library:

    (1) Cleave DNA with restriction enzyme - manageable size;

    (2) All these random DNA fragments inserted into plasmids, using DNA ligase, (to get recombinant DNA molecules;)

本次内容到此结束，感谢阅读！下一期内容将讲述下一种技术：DNA测序 与 聚合酶链式反应PCR。

作者：離久-張所長