质谱应用教程-如何调高 QTof 的分辨率
目的
增加 QTof 的质谱分辨率。
环境
MassLynx 4.1
Synapt G2-Si
Synapt G2-S
Synapt G2
Xevo G2 XS
Xevo GS S
Xevo G2
Xevo QTof
步骤
在 Tune 页面上，从设置下拉菜单中选择 System View（系统视图）。密码：access。
选择 Acquisition Settings（采集设定）。您将在左下角看到分辨率。这是此模式的预期分辨率。记下该分辨率并退出此窗口。
放大锁定质量数化合物的峰。
通过降低 LockSpray 毛细管电压，将峰计数调整为中等至低 e4 计数。或者进入 pDRE Test（pDRE 测试）下的 System 2（系统 2）选项卡，选择 Attenuate（衰减）并调整 Transmission（传输），直到调整为低 e4 计数。注意：如果您愿意，请右键单击 Tune 页面中的峰，然后选择 Intensity（强度）> 选择 Normalize Data（归一化数据）。这样可以在调节分辨率时查看强度是否增加或降低。
放大峰，以便轻松查看峰形。
打开 System 2（系统 2）页面，使用向左或向右箭头键调节反射器网格reflectron grid电压（单击黑色数字右侧的滑块，然后使用键盘上的箭头键进行调节）。调节以获取最佳分辨率、峰形和灵敏度，分辨率优先级最高。
接下来打开 System 1（系统 1）页面，使用向左或向右箭头键调节推动pusher offset电压偏置值（单击黑色数字右侧的滑块，然后使用键盘上的箭头键进行调节）。调节获取最佳分辨率、峰形和灵敏度，对称峰形优先级最高。
How to tune up the resolution on a QTof - WKB13440
Last updatedDec 21, 2021
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Article number: 13440
OBJECTIVE or GOAL
Increase the mass spectral resolution on a QTof.
ENVIRONMENT
MassLynx 4.1
SYNAPT G2 Si
SYNAPT G2 S
SYNAPT G2
Xevo G2 XS
Xevo GS S
Xevo G2
Xevo QTof
PROCEDURE
On the Tune page, from the setup drop-down menu, select System View. Password: "access".
Select Acquisition Settings. On the bottom left, you will see Resolution. This is the expected resolution for this mode. Take note of the resolution and exit this window.
Zoom in on lockmass peak.
Adjust counts to mid to low e4 counts by dropping the LockSpray capillary voltage. Or going to the System 2 tab under pDRE Test, select Attenuate, and adjust the Transmission until you have low e4 counts. Note: If you are comfortable doing so, right-click the peak in the tune page and select Intensity > select Normalize Data. This allows you to see if the intensity is going up or down as you tune the resolution.
Zoom in on the peak so that you can easily see the peak shape.
Go to the System 2 page and adjust the reflectron grid by using the left or right arrow key (click on the slider to the right of the number in black, and then use the arrow keys on the keyboard). Tune for best resolution, peak shape, and sensitivity, with resolution being the highest priority.
Now go to the System 1 page and adjust the pusher offset by using the left or right arrow key (click on the slider to the right of the number in black, and then use the arrow keys on the keyboard). Tune for best resolution, peak shapem and sensitivity, with symmetrical peak shape being the highest priority.
