多功能艾美捷钙网蛋白（CRT）单克隆抗体

The multifunctional, multi -compartmental protein Calreticulin (Crt) functions as a soluble molecular chaperone of new or misfolded proteins, as well as a Ca2+-binding protein. Most abundant in the ER lumen, Crt expression also occurs in other membrane-bound organelles, the cell surface, and extracellularly. Also known as CRP-55, calregulin and HACBP (high affinity calcium-binding protein), Crt contains the ER-retrieval sequence, KDEL, and is the solub le paralog of the ER membrane protein Calnexin (Cnx). Crt's three domains include a 180 residue N-terminal domain, a proline-rich Pdomainresidues 189 -288) that binds Ca2+ with high affinity and shares homology with Cnx and calmegin, and a 110 residue C-terminal domain that binds Ca2+ with low affinity but high capacity. The P-domain may interact with the co-chaperone ERp57 (Grp58), a thiol reductase. The NMR structure of the P -domain consists of an extended hairpin that appears to form a curved protrusion from the Crt core domain. Both Crt and its membrane bound homolog CNX interact with proteins and glycoproteins possessing monoglucosylated N -glycans. The Crt/Cnx cycle promotes correct folding, inhibits aggregation of folding intermediates, blocks premature oligomerization, regulates ER degradation, and prevents incompletely folded glycoproteins from exiting to the Golgi complex. Crt also appears to function as an auto-antigen in systemic lupus erythematosus, rheumatoid arthritis, celiac disease, complete congenital heart block, and halothane hepatitis. A diversity of additional functions attributed to Crt includes adhesion, blood function, and cardiac and neuronal development gene expression.

 

多功能、多区室蛋白钙网蛋白（Crt）可作为新的或错误折叠的蛋白质的可溶性分子伴侣，以及Ca2+结合蛋白。Crt 表达在 ER 管腔中最为丰富，也发生在其他膜结合细胞器、细胞表面和细胞外。Crt 也称为 CRP-55、钙调节蛋白和 HACBP（高亲和力钙结合蛋白），含有 ER 检索序列 KDEL，是 ER 膜蛋白钙连接蛋白 （Cnx） 的可溶物。

 

CRT 似乎也可作为系统性红斑狼疮、类风湿性关节炎、乳糜泻、完全性先天性心脏传导阻滞和氟烷肝炎的自身抗原发挥作用。Crt 的多种附加功能包括粘附、血液功能以及心脏和神经元发育基因表达。

艾美捷钙网蛋白（CRT）单克隆抗体（ADI-SPA-601PE-D）基本参数：

克隆： FMC 75

宿主： 鼠

同种型： 免疫球蛋白G1

免疫原： 重组钙网蛋白。

源： 从腹水中净化

物种反应性： 人类

猴子

应用： 流式细胞术

推荐稀释度/条件： 流式细胞术 （1：100）

建议的稀释度/条件可能不适用于所有应用。

应用说明： 预测分子量为~63kDa。

纯度细节： 蛋白G亲和纯化。

配方： 液。在PBS中，pH 7.2，含有0.09%叠丨氮丨化钠。

处理： 避免冻融循环。避光。

航运： 蓝冰不结冰

长期储存： +4°C

 

钙网蛋白（CRT）单克隆抗体部分文献引用：

CD47 Blockade Leads to Chemokine-Dependent Monocyte Infiltration and Loss of B Cells from the Splenic Marginal Zone: Y. Y. Yiu, et al.; J. Immunol. 208, 1371 (2022);

IMMUNEPOTENT CRP induces DAMPS release and ROS-dependent autophagosome formation in HeLa and MCF-7 cells: A.C. Martinez-Torres, et al.; BMC Cancer 20, 647 (2020); Full Text

Programmed cell removal by calreticulin in tissue homeostasis and cancer: M. Feng, et al.; Nat. Commun. 9, 3194 (2018);

CD47 Blockade Leads to Chemokine-Dependent Monocyte Infiltration and Loss of B Cells from the Splenic Marginal Zone: Y. Y. Yiu, et al.; J. Immunol. 208, 1371 (2022);

IMMUNEPOTENT CRP induces DAMPS release and ROS-dependent autophagosome formation in HeLa and MCF-7 cells: A.C. Martinez-Torres, et al.; BMC Cancer 20, 647 (2020); Full Text

Programmed cell removal by calreticulin in tissue homeostasis and cancer: M. Feng, et al.; Nat. Commun. 9, 3194 (2018);

 

钙网蛋白（CRT）单克隆抗体相关研究：

钙网蛋白单克隆抗体 （FMC 75）ADI-SPA-601-F

钙网蛋白单克隆抗体 （FMC 75）（DyLight™ 488 偶联物） ADI-SPA-601-488-F

小鼠 IgG1 同种型对照，单克隆抗体 （MOPC-21）（PE 偶联物） ADI-SAB-600PE-F