免疫组化笔SuperHT PAP Pen，保存珍贵的抗体和试剂

Cat.# Product Name Unit Size

Catalog Number:

Mini Super HT Pap Pen: 22005

Super HT Pap Pen: 22006

Unit Size: 1 pen

Size:

Mini Super HT Pap Pen: 2.5 mm tip, ~400 applications Super HT Pap Pen: 4 mm tip, ~800 applications

Number of applications varies depending on barrier size

Storage and handling: Store at room temperature. The pens contain organic solvents. Avoid skin and eye contact and keep away from open flame. Cap tightly after each use.

Product Description

Super HT Pap Pens are used to create hydrophobic barriers around tissue sections on glass slides to hold staining solutions in place on the slide. This allows the conservation of antibody staining solution or staining of two sections on the same slide with different solutions. The Mini Super HT Pap Pen has a fine point that is useful for separating multiple sections on the same slide. Super HT Pap Pen barriers

are insoluble in aqueous buffers, detergents, alcohol and acetone, but can be removed with xylene. Barriers are stable at temperatures up to 120oC.

Directions

Before first use, fill the tip with barrier fluid. Press the tip down on a clean glass microscope slide to open the tip valve (Figure 1). Keep pressing down until you see the tip fill completely with green liquid. Stop pressing as soon as the tip is completely filled.

Barriers should be created after deparaffinization and rehydration for paraffin sections. For frozen sections, create barriers when slides are dry, before the first buffer incubation step. To create a barrier, dry the area around the section with a cotton swab if necessary. Draw an unbroken circle of Pap Pen fluid around the section (Figure 2). Do not press the valve down while drawing the barrier. Take care to prevent the Pap Pen fluid from touching the tissue section. Let the barrier dry 15-30 seconds before immersing slides or adding buffer. Dry the area immediately outside the barrier if necessary and add just enough buffer to fill the barrier without overflowing. A square of Parafilm can be placed on top of the section to spread the buffer evenly and prevent evaporation. For overnight incubations, slides should be placed in a humidified chamber to prevent evaporation.

艾美捷免疫组化笔 SuperHT PAP Pen#BTM-22006使用方法：

图1：第一次使用前先填充笔尖。A、 笔尖包装干燥。B、 新闻提示向下靠在显微镜载玻片上，直到尖端完全充满绿色液体（C）。

图2：制造障碍。A、 在剖面周围画一个不间断的圆圈用巴氏笔。B、 将缓冲液移液到屏障中。添加足够的缓冲区填充屏障而不溢出（C）。

免疫组化笔 SuperHT PAP Pen特征：

在载玻片上的组织样本周围绘制防水屏障

保存珍贵的抗体和试剂

屏障不溶于水性缓冲液、洗涤剂、酒精和丙酮

阻隔层在高达 120°C 的温度下保持稳定

笔含有疏水性有机溶剂

免疫组化笔 SuperHT PAP Pen规格：

迷你超高温巴氏笔：2.5毫米笔尖，约400次使用

Super HT Pap笔：4毫米笔尖，约800次使用

应用程序的数量因屏障大小而异