艾美捷PolyMag CRISPR试剂盒,高转染效率无毒性
艾美捷PolyMag CRISPR试剂盒#PNC40200高转染效率无毒性,灵敏性高、特异性强,用途范围广,具体特色如下:
• 即使在原代细胞和难以转染的细胞上也能高效递送 CRISPR/Cas9 系统• 非常适合共转染 pDNA/pDNA、pDNA/gRNA、pDNA/mRNA
• 针对含有 Cas9 的大质粒 DNA 进行了优化,并引导 RNA
• 低细胞毒性 - 能够以更少的细胞开始实验
PolyMag CRISPR kit is the only magnet assisted transfection reagent optimized to deliver high level of plasmid DNA and/or mRNA expressing Cas9 and guide RNA (gRNA). This reagent is based on our proprietary Magnetofection™ technology. The nanoparticles are composed of iron oxide magnetic core coated with cationic molecules. For your gene editing experiments, this reagent provides high transfection efficiency without toxicity.
Benefits:
• High efficiency delivery of CRISPR/Cas9 system even on primary and hard to transfect cells
• Ideal for co-transfection of pDNA/pDNA, pDNA/gRNA, pDNA/mRNA
• Optimized for large plasmid DNA containing Cas9, and guide RNA
• Low cell toxicity - enable to start your experiment with less cell
用于CRISPR Cas9实验的其他产品:
ViroMag CRISPR提高CRISPR/Cas9病毒的转导效率
RmesFect CRISPR 用于 mRNA 转染
ProDeliverIN CRISPR用于 Cas9 蛋白递送
储存: +4°C
运输条件:室温

Figure1 : PolyMag CRISPR co-transfection efficiency for CRISPR-Cas9 experiments. NIH-3t3, HeLa and MDCK cells were plated in 24-well plates in complete medium at the optimum density of 50-70% confluence at the time of transfection. Cells were transfected with a GFP-Cas9 plasmid and a RFP pDNA and then incubated overnight at 37oC. GFP and RFP expression was monitored 24h after transfection by fluorescence microscopy.