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艾美捷PolyMag CRISPR试剂盒,高转染效率无毒性

2023-04-07 15:52 作者:艾美捷  | 我要投稿

艾美捷PolyMag CRISPR试剂盒#PNC40200高转染效率无毒性,灵敏性高、特异性强,用途范围广,具体特色如下:

• 即使在原代细胞和难以转染的细胞上也能高效递送 CRISPR/Cas9 系统• 非常适合共转染 pDNA/pDNA、pDNA/gRNA、pDNA/mRNA

• 针对含有 Cas9 的大质粒 DNA 进行了优化,并引导 RNA

• 低细胞毒性 - 能够以更少的细胞开始实验

 

PolyMag CRISPR kit is the only magnet assisted transfection reagent optimized to deliver high level of plasmid DNA and/or mRNA expressing Cas9 and guide RNA (gRNA). This reagent is based on our proprietary Magnetofection™ technology. The nanoparticles are composed of iron oxide magnetic core coated with cationic molecules. For your gene editing experiments, this reagent provides high transfection efficiency without toxicity.

 

Benefits:

•    High efficiency delivery of CRISPR/Cas9 system even on primary and hard to transfect cells

•    Ideal for co-transfection of pDNA/pDNA, pDNA/gRNA, pDNA/mRNA

•    Optimized for large plasmid DNA containing Cas9, and guide RNA

•    Low cell toxicity - enable to start your experiment with less cell

 

用于CRISPR Cas9实验的其他产品:

ViroMag CRISPR提高CRISPR/Cas9病毒的转导效率

RmesFect CRISPR 用于 mRNA 转染

ProDeliverIN CRISPR用于 Cas9 蛋白递送

储存: +4°C

运输条件:室温

 

 


Figure1 : PolyMag CRISPR co-transfection efficiency for CRISPR-Cas9 experiments. NIH-3t3, HeLa and MDCK cells were plated in 24-well plates in complete medium at the optimum density of 50-70% confluence at the time of transfection. Cells were transfected with a GFP-Cas9 plasmid and a RFP pDNA and then incubated overnight at 37oC. GFP and RFP expression was monitored 24h after transfection by fluorescence microscopy.


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