【MIT细胞生物学】复习大纲 04-06
学习顺序与主题 LEARNING SEQUENCES AND TOPICS
4．运输机制
5. 细胞隔间运输
6. 胞吞与胞吐
目标 GOALS
Review how the steps of translation, post-translational modifications, and folding map to distinct locations in the cell.
了解翻译、翻译后修饰和折叠如何映射到细胞的不同位置。
Understand the processes involved in discrete steps of protein maturation and transport.
了解蛋白质成熟和运输的独立步骤及其所涉及的过程
Understand how specific molecular machinery is essential to protein transport across cellular compartments.
了解特定的分子机制为何对跨细胞隔间的蛋白质转运至关重要。
Detail the molecular mechanisms of protein trafficking to membranes.
详细说明蛋白质向膜运输的分子机制。
你应该能做到 You should be able to
1. Identify the cellular locations of different biochemical and spatial steps during protein trafficking.
2. Interpret the results of a pulse-chase experiment.
3. Design an experiment to determine the location of the signal for protein transport.
4. Explain the significance of the signal sequence.
5. Detail the models (cotranslational vs. post-translational translocation) for protein entry into the secretory pathway.
6. Describe the structure and function of the signal recognition particle (SRP), the SRP receptor, and the Sec61 channel (translocon)
7. Explain how protein folding and covalent modifications are interdependent.
8. Summarize general features of protein glycosylation throughout the secretory pathway.
9. Identify the functions of different classes of proteins, such as chaperones and coats, in protein trafficking.
10. Propose a mechanism for how proteins move between cellular compartments depending on their final cellular destination.
11. Describe the GTPase, GAP, and GEF mechanisms important in protein trafficking.
12. Propose a role for SNAREs and Rabs in vesicle movement.
13. Explain the roles of protein trafficking, exocytosis, and endocytosis in regulating cellular processes.
14. Summarize the mechanism of clathrin-mediated endocytosis, as exemplified by cholesterol uptake.
15. Describe how viruses can utilize host cell endocytosis and exocytosis pathways throughout their life cycles.
