学习顺序与主题 LEARNING SEQUENCES AND TOPICS

1. 生物膜的成分

1.1 细胞质膜 吾即屏障

1.2 磷脂和磷脂双分子层

1.3 膜蛋白的特性

1.4 使用FRAP检测流动性

2. 脂质的异质性与不对称性

2.1 脂质的异质性

2.2 质膜中的其它脂质

2.3 脂质的不对称性

3. 蛋白质的不对称性及其预测

3.1 蛋白质的不对称性

3.2 蛋白质的取向预测

3.3 表位预测蛋白取向

目标 GOALS

1. Understand the contribution of specific proteins and lipids to the distinct properties of biological membranes. 

了解特定蛋白质和脂质对生物膜独特性质的贡献。

2. Understand experimental Approaches utilized by scientists to study the mobility, orientation, and function(s) of membrane-associated proteins.

了解科学家用来研究膜相关蛋白质的迁移、取向和功能的实验方法。

3. Appreciate the properties conferred by membrane fluidity, heterogeneity, and asymmetry.

欣赏膜流动性、异质性和不对称性所赋予的特性。

你应该能做到 You should be able to

1. Assess why lipids are central to the structure of biological membranes.

为什么脂质是生物膜结构的核心。

2. Compare and contrast lipid components of the phospholipid bilayer.

对比磷脂双层的脂质成分。

3. Describe the functional significance of lipid heterogeneity. 

描述脂质异质性的功能意义。

4. Explain the mechanism by which cells maintain the concentration differences of small molecules across biological membranes. 

解释细胞维持小分子跨生物膜浓度差异的机制。

5. Distinguish the topographical classifications of membrane proteins: type I, II, and multipass transmembrane, integral, peripheral, and lipid-anchored membrane proteins.

区分膜蛋白的拓扑类型：I 型、II 型和多次跨膜蛋白、整合膜蛋白、外周膜蛋白和脂锚定膜蛋白。

6. Contrast the properties and Applications of non-ionic and ionic detergents.

对比非离子和离子型洗涤剂的性能和应用。

7. Analyze results from Fluorescent Recovery After Photobleaching (FRAP) experiments.

分析光脱色荧光恢复技术 (FRAP) 的实验结果。

8. Propose an experiment to determine the orientation of a membrane protein.

设计实验以确定膜蛋白方向。

9. Predict transmembrane domains based on primary structure.

根据一级结构预测跨膜结构域。

10. Distinguish different kinds of channels, transporters, and pumps. 

区分不同种类的通道、转运蛋白和离子泵