【文献速递】【NBE】【2022年】【2-3月】
声明：本专栏主要对生命科学领域的一些期刊文章标题进行翻译，所有内容均由本人手工整理翻译。由于本人专业为生物分析相关，其他领域如果出现翻译错误请谅解。
Prime editing of pathogenic mutations in mouse eyes and livers
小鼠眼睛和肝脏致病突变的初始编辑
This issue highlights microfluidic sorting for the recovery of potent tumour-infiltrating lymphocytes, the generation of potent CAR T cells in one day, systemic anticancer immunity via intratumourally injected alum-tethered cytokines, synergistic checkpoint blockade and radiotherapy–radiodynamic therapy via nanoscale metal–organic frameworks, in vivo genome editing via disruption of Cas9 activity in the liver, a brain-wide Cas9-mediated therapy for familial Alzheimer’s disease, prime editing for the correction of mutations in adult mice, the restoration of dystrophin expression by suppressing a nonsense mutation via unnatural amino acids, and the reversal of RNA toxicity in myotonic dystrophy via a decoy RNA-binding protein.
本期重点介绍了用于恢复强效肿瘤浸润淋巴细胞的微流体分选、一天内产生强效CAR T细胞、通过肿瘤内注射明矾束缚细胞因子的全身抗癌免疫、协同检查点阻断和通过纳米级金属-有机物的放射疗法-放射动力学疗法框架，通过破坏肝脏中的Cas9活性进行体内基因组编辑，全脑Cas9介导的家族性阿尔茨海默氏病治疗，用于校正成年小鼠突变的初步编辑，通过抑制无义突变恢复抗肌萎缩蛋白表达非天然氨基酸，以及通过诱饵RNA结合蛋白逆转强直性肌营养不良症中的RNA毒性。
1.Efficient recovery of potent tumour-infiltrating lymphocytes through quantitative immunomagnetic cell sorting.
通过定量免疫磁细胞分选有效恢复潜在的肿瘤浸润淋巴细胞。
2.Rapid manufacturing of non-activated potent CAR T cells.
快速生产非激活的有CAR T细胞。
3.Intratumourally injected alum-tethered cytokines elicit potent and safer local and systemic anticancer immunity.
瘤内注射明矾束缚细胞因子可引发有效且更安全的局部和全身抗癌免疫。
4.Synergistic checkpoint-blockade and radiotherapy–radiodynamic therapy via an immunomodulatory nanoscale metal–organic framework.
通过免疫调节纳米级金属有机框架协同检查点封锁和放射疗法-放射动力学疗法。
5.Augmented lipid-nanoparticle-mediated in vivo genome editing in the lungs and spleen by disrupting Cas9 activity in the liver.
通过破坏肝脏中的Cas9活性，增强脂质纳米颗粒介导的肺和脾脏体内基因组编辑。
6.Brain-wide Cas9-mediated cleavage of a gene causing familial Alzheimer’s disease alleviates amyloid-related pathologies in mice.
全脑Cas9介导的导致家族性阿尔茨海默氏病的基因切割减轻了小鼠的淀粉样蛋白相关病症。
7.Application of prime editing to the correction of mutations and phenotypes in adult mice with liver and eye diseases.
主要编辑在校正患有肝病和眼病的成年小鼠的突变和表型中的应用。
8.Restoration of dystrophin expression in mice by suppressing a nonsense mutation through the incorporation of unnatural amino acids.
通过掺入非天然氨基酸抑制无义突变，恢复小鼠抗肌萎缩蛋白的表达。
9.Reversal of RNA toxicity in myotonic dystrophy via a decoy RNA-binding protein with high affinity for expanded CUG repeats.
通过对扩增的CUG重复具有高亲和力的诱饵RNA结合蛋白逆转强直性营养不良症中的 RNA毒性。
血清样本中卵巢癌的光谱指纹图谱
This issue highlights the performance of paper-based cell-free diagnostics for the detection of the Zika and chikungunya viruses in serum samples, a method for the tracking of thousands of mutations with fewer reads per locus than conventional hybrid-capture duplex sequencing, that ovarian cancer can be predicted via a fingerprint obtained via machine learning from the emissions of carbon-nanotube sensors in serum samples, fast and sensitive electromechanical and biochemical methods for the detection of biomolecules, cell-free diagnostics via multi-arm junction RNAs that execute molecular logic, and the urinary detection of early responses to checkpoint blockade via protease-cleaved antibody-conjugated sensors.
本期重点介绍了基于纸质的无细胞诊断检测血清样本中寨卡病毒和基孔肯雅病毒的性能，这是一种跟踪数千个突变的方法，每个位点的读取次数少于传统的杂交捕获双重测序，卵巢癌可以通过机器学习从血清样本中利用碳纳米管传感器获得的指纹来预测癌症，用于检测生物分子的快速灵敏的机电和生化方法，通过执行分子逻辑的多臂连接RNA进行无细胞诊断，以及通过蛋白酶切割的抗体偶联传感器检测对检查点封锁的早期反应的尿液检测。
1.Field validation of the performance of paper-based tests for the detection of the Zika and chikungunya viruses in serum samples.
在血清样品中检测寨卡病毒和基孔肯雅病毒的基于纸质测试的性能的现场验证。
2.Massively parallel enrichment of low-frequency alleles enables duplex sequencing at low depth.
低频等位基因的大量平行富集可以在低深度下进行双链测序。
3.Detection of ovarian cancer via the spectral fingerprinting of quantum-defect-modified carbon nanotubes in serum by machine learning.
通过机器学习，通过量子缺失改性的碳纳米管的光谱指纹识别卵巢癌。
图1. OOC-DNA纳米传感阵列。a。有机颜色中心-DNA（Organic colour centres，OCC-DNA）纳米传感元素。显示的是带有3,4,5-三氟芳基OCC的 ss(GT)15 DNA包裹(6,5)-碳纳米管。b。从OCC和ssDNA组件构建OCC-DNA阵列。
4.Rapid and ultrasensitive electromechanical detection of ions, biomolecules and SARS-CoV-2 RNA in unamplified samples.
在未扩增样品中的离子，生物分子和SARS-COV-2 RNA的快速和超敏机电学检测。
a．通过MEMS或者NEMS进行生物检测的原理图。b. MolEMS及其静电驱动的原理图。单链DNA悬臂顶端结合了一个探针，用于特异的生物识别。E代表电场。c. MolEMS g-FET的设备配置。d,e. 设备的照片（d）和光学显微镜（e）图片。f. 固定在MolEMSs上石墨烯的AFM图片（在1倍的TM缓冲液中）。颜色标尺表明扫描面积的高度。g-i, 使用Cy3-17bp-15T MolEMSs石墨烯的荧光图片。Vlg是0.9V（g），0V（h）和-1.1V（i）Cy3偶联在ssDNA悬臂的顶端作为荧光染料。比例尺，100 nm （f）和 30 μm （e,g–i）.
5.Fast and sensitive detection of SARS-CoV-2 RNA using suboptimal protospacer adjacent motifs for Cas12a.
使用次优原型间隔子相邻基序CAS12a对SARS-COV-2 RNA的快速和敏感检测。
6.Multi-arm RNA junctions encoding molecular logic unconstrained by input sequence for versatile cell-free diagnostics.
多臂RNA连接编码分子逻辑的编码无细胞诊断的输入序列不受限制的分子逻辑。
7.Urinary detection of early responses to checkpoint blockade and of resistance to it via protease-cleaved antibody-conjugated sensors.
通过蛋白酶切割的抗体偶联传感器对检查点阻断的早期反应以及对其抗性的尿液检测。
