美国密歇根大学张可博士-基于RNA 干扰的生物农药的环境归趋——生物风险评估一瞥

扫码二维码观看直播

入会暗号   

直播时间：2023年3月5日 9:00am（北京时间）

Zoom会议ID：816 9975 7155

Bilibili链接：

https://live.bilibili.com/25002335?broadcast_type=0&;is_room_feed=1&spm_id_from=333.999.0.0（生态环境健康EEH）

本期主题

Environmental fate of RNA interference biopesticides – a glance at biorisk assessment

基于RNA 干扰的生物农药的环境归趋

——生物风险评估一瞥

本期主持：

何益得 副教授

南京工业大学

EEH期刊青年编委

特邀主讲：

Ke Zhang（张可） 博士 

美国密歇根大学

   张可博士的研究方向是环境生物化学，以应对自然和工程生物污染物带来的环境挑战。他的最终研究目标是预测生物污染物在环境系统中的行为，并优化策略以将其对人类和生态系统健康造成的风险降至最低。他在圣路易斯华盛顿大学获得了博士学位，研究重点是基于 RNA 干扰的生物农药的环境归趋。目前，他是密歇根大学的博士后研究员，主要研究流感病毒在室内环境中的归趋。

报告摘要

  In recent years, scientists have developed RNA interference crops that express double-stranded RNA (dsRNA) biopesticides in their tissues. While these genetically modified crops have shown to be effective in controlling pests, they also release dsRNA biopesticides to receiving environments and pose potential ecological risks to non-target organisms depending on the environmental concentration of dsRNA biopesticides. My work aimed to enable the accurate ecological risk assessments of dsRNA biopesticides. Firstly, I developed a new method using quantitative reverse transcription polymerase chain reaction (RT-qPCR) to measure dsRNA in agricultural soils at environmentally relevant concentrations. Secondly, I characterized the chemical stability of dsRNA in aqueous solutions by examining the impacts of the duplex structure of dsRNA on alkaline hydrolysis. Finally, I elucidated a novel abiotic reaction by which RNA, but not DNA, degraded upon adsorption to minerals in soils and sediments under environmentally relevant physicochemical conditions. These studies advance our understanding of the fate of dsRNA biopesticides in receiving environments and contribute to the ecological risk assessment of dsRNA biopesticides.