吐温/聚山梨酯 20和80之间的区别是什么?
A Discussion of Protein Research
20和80之间的区别是什么?
As a non-ionic viscous liquid, tweens are a class of emulsifiers used in pharmaceuticals and food preparation.
作为一种非离子粘性液体，吐温是一类用于药品和食品制备的乳化剂。
Tween® 20 and Tween® 80 are both biocompatible surfactants used in food, biotechnical, and pharmaceutical applications. However, despite their similar uses, there are differences between the two types of tweens.
Tween 20和Tween 80都是生物相容性表面活性剂，用于食品、生物技术和制药应用。然而，尽管它们的用途相似，但两种类型的补间之间存在差异。
What is Tween®?
Otherwise known as polysorbates, tweens are typically used as a surfactant for dispersing hydrophobic particles in aqueous solutions, as well as a non-ionic detergent for selective protein extraction and isolation of nuclei from mammalian cells.
也称为聚山梨酯，吐温通常用作表面活性剂，用于分散水溶液中的疏水粒子，以及非离子洗涤剂，用于选择性蛋白质提取和分离哺乳动物细胞的细胞核。
Tweens are a condensate of sorbitol fatty acid ester and ethylene oxide, and either soluble or dispersible in water but differ widely in organic and oil solubilities.
吐温是山梨醇脂肪酸酯和环氧乙烷的凝结物，可溶于水或分散于水，但在有机溶解度和油溶解度方面差别很大
What is Tween® 20?
Tween® 20, or Polysorbate 20, is a popular choice for biochemical applications. With a hydrophobic dodecanoic tail, it attached to 20 repeat units of polyethylene glycol and distributed across four different chains.
吐温20，或聚山梨酯20，是一个流行的选择生化应用。它有一个疏水的十二烷尾巴，附着在20个重复单元的聚乙二醇上，分布在四个不同的链上。
As a non-ionic surfactant, Polysorbate 20 has a molecular weight of 1,225 daltons, assuming 20 ethylene oxide units, one sorbitol, and one lauric acid as the primary fatty acid.
聚山梨醇20作为一种非离子表面活性剂，其分子量为1225道尔顿，假定环氧乙烷20个单位，山梨醇1个单位，月桂酸1个单位为主要脂肪酸
The ethylene oxide subunits are responsible for the hydrophilic nature of the surfactant, while the hydrocarbon chains provide the hydrophobic environment. Ethylene oxide polymers attach to the backbone ring, which is formed by sorbitol.
环氧乙烷亚基负责表面活性剂的亲水性，而碳氢链提供疏水环境。环氧乙烷聚合物附着在山梨醇形成的主环上。
It is also miscible in water (100 mg/ml) and yields a clear, yellow solution. It is practically insoluble in liquid paraffin and fixed oils, and also miscible in alcohol, dioxane, and ethyl acetate.
它在水（100mg / ml）中也会混溶，得到清澈的黄色溶液。 它实际上不溶于液体石蜡和固定油，也可以在醇，二恶烷和乙酸乙酯中混溶。
What is Tween® 80?
Tween® 80 is a non-ionic detergent for solubilizing membrane proteins during the isolation of membrane-protein complexes, and is available with ultra-low concentrations of contaminating peroxides, aldehydes, salts, and carbonyl compounds.
吐温80是一种非离子洗涤剂，用于在膜蛋白复合物分离过程中溶解膜蛋白，可用于污染物过氧化物、醛、盐和羰基化合物的超低浓度。
Tween 80 is also a polyethylene sorbitol ester, otherwise known as Polysorbate 80 or polyoxyethylene sorbitan monooleate. It has a molecular weight of 1.31 kDa and works well as a stabilizer and emulsifier, primarily in cosmetics, pharmaceuticals, and food products.
吐温80也是一种聚乙烯山梨醇酯，或称为聚山梨醇80或聚氧乙烯山梨醇单油酸酯。它的分子量为1.31 kDa，可以作为稳定剂和乳化剂，主要用于化妆品、药品和食品。
Tween 80 is also miscible in water (0.1 ml/ml) and yields a clear, yellow solution. It is also miscible with alcohol, dioxane, and ethyl acetate, and is practically insoluble in liquid paraffin and fixed oils.
吐温80也在水（0.1ml / ml）中混溶，得到澄清的黄色溶液。 它也可与乙酸醇，二恶烷和乙酸乙酯混溶，实际上不溶于液体石蜡和固定油。
What is the Difference Between Tween 20 and Tween 80?
Tween 20 and Tween 80 are both used in food, biotechnical, and pharmaceutical applications. However, they are present in different types of applications.
Tween 20和Tween 80都用于食品、生物技术和制药应用。然而，它们存在于不同类型的应用程序中。
Tween 20 is a common component in the following applications:
吐温20是以下应用中的常见成分：
The pre-extraction of membranes to remove peripheral proteins （预萃取膜去除外周蛋白）
Membrane-based immunoassays （基于膜的免疫测定）
Lysing mammalian cells （裂解哺乳动物细胞）
PCR (polymerase chain reaction（聚合酶链反应）
ELISA (enzyme-linked immunosorbent assay) （酶联免疫吸附试验）
Western blot analysis 印迹分析
Due to its drying property, Tween 80 is typically present in ice cream. It is also widely utilized in biochemical applications, including:
由于其干燥性，吐温80通常存在于冰淇淋中。 它也广泛用于生化应用，包括：
Isolating nuclei from mammalian cell lines（从哺乳动物细胞中分离核）
Growing of tubercle bacilli（生长结节杆菌）
Selective protein extraction (选择性蛋白质提取)
Emulsifying and dispersing substances in medicinal and food products (药品和食品中的乳化和分散物质)
As a hydrophilic emulsifier in the preparation of W1/O/W2 emulsion (作为制备W1 / O / W2乳液的亲水性乳化剂)
