中英文说明丨抗原修复液（BTM-22030）方案

AntiFix™ Universal Antigen Retrieval Buffer, 10X

Catalog Number: 22030-50mL, 22030-500mL

Unit Size: 50 mL, 500 mL

Storage and Handling

Store at room temperature. Product is stable for at least 12 months from date of receipt when stored as recommended. The buffer is non-toxic and non-flammable.

Product Description

AntiFix™ Universal Antigen Retrieval Buffer is designed for heat-induced epitope retrieval (HIER) of formalin-fixed, paraffin embedded (FFPE) tissues. The buffer uses a proprietary reagent that catalyzes the reversal of formaldehyde cross-links in FFPE tissue to restore antigen reactivity.

Fixation of tissue with formalin (formaldehyde) preserves tissue morphology, but the cross-linking of proteins by formaldehyde can modify antibody binding sites or epitopes so they are no longer detectable by immunohistochemistry. Traditionally,epitope reactivity is recovered by using protease treatment, or by heating slides in either low pH citrate buffer or high pH Tris buffer. The optimal method for antigen retrieval varies for different epitopes, requiring multiple buffers to be used for different targets.

AntiFix™ Universal Antigen Retrieval Buffer has been formulated to provide excellent heat unmasking for a wide variety of targets at neutral pH, reducing the need for buffer optimization. It has been validated in immunofluorescence staining of FFPE tissue with our next-generation fluorescent CF® dyes using HRPmediated tyramide signal amplification.

 

AntiFix Universal Antigen Retrieval Buffer可在中性pH条件下对多种靶标进行出色的解蔽。经验证，它可用于使用HRP介导的酪胺信号放大的下一代荧光CF®染料对FFPE组织进行荧光染色。50 mL 10 X缓冲液。

 

艾美捷抗原修复液#BTM-22030特征：

中文名称:抗脂多糖核心单克隆抗体（克隆号WN1 222-5） 现货

英文名字:Lipopolysaccharide Core， mAb WN1 222-5

Hycult编号:HM6011

规格:50ug

应用类型:FS、IA、P、WB

免疫原:小鼠来源的108sup> heat-killed bacteria

来源宿主:小鼠

反应性:抗脂多糖核心

保存建议:该产品为液体形式，推荐4℃保存，可保存1年。

 

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