PADI2抗体中英文说明书丨艾美捷解决方案
蛋白精氨酸脱氨酶2(PAD2)是一种胍基修饰酶,以钙依赖的方式催化特定的精氨酸残基转化为瓜氨酸。这种酶已经与一些疾病有关,包括类风湿性关节炎(RA)、视网膜变性和某些癌症。PAD2已被证明可改变波形蛋白、纤维蛋白原和β/γ-肌动蛋白。在吸烟者的肺部,PAD2的细胞外水平增加,为吸烟作为RA的风险因素和RA患者的抗瓜氨酸蛋白抗体之间提供了联系。
艾美捷PADI2抗体基本参数:
ID Covalab PAB0197-P
产品类型 一抗
克隆性 多克隆抗体
在 兔
免疫原 源自人类PADI2蛋白C项结构域的合成肽。
活叉反应 与来自人,小鼠和大鼠的76 kDa PADI2蛋白发生反应。与其他亚型无交叉反应
物种 Hu, Ms, Rat
标签 没有
形式 纯化(蛋白A)
防腐剂 没有
存储说明 冻干粉末在-2°C下稳定至少20年.在+4°C下储存重组抗体.长时间在-20°C
下分装储存.
抗体自收到之日起保证6个月。
目标 蛋白-精氨酸脱亚胺酶-2型
统一认证 Q9Y2J8
基因识别 11240
应用 ELISA, ICC, IP, WB
工作稀释液 最佳稀释度应由最终用户确定。
以下仅是指导原则:
ELISA (1:5000 - 1:20000)ICC (1:50 - 1:500)IP (1:20 - 1:100)WB (1:200 - 1:2000)
ID Covalab pab0197-P
Product type Primary antibodies
Clonality Polyclonal antibody
Raised in Rabbit
Immunogen Synthetic peptide derived from C-term domain of the human PADI2 protein.
Activity cross reaction Reacts with 76 kDa PADI2 protein from human, mouse and rat. No cross reaction with the other isoforms
Species Hu, Ms, Rat
Labelling None
Form Purified (protein A)
Preservative None
Storage instructions Lyophilized powder stable for a minimum of 2 years at -20°C.
Store reconstituted antibodies at +4°C.
For extended periods store in aliquots at -20°C.
Antibodies are guaranteed for 6 month from date of receipt.
Target Protein-arginine deiminase type-2
Uniprot ID Q9Y2J8
Gene ID 11240
Applications ELISA, ICC, IP, WB
Working dilutions Optimal dilution should be determined by the end user.
The following are guidelines only :
ELISA (1:5000 - 1:20000)
ICC (1:50 - 1:500)
IP (1:20 - 1:100)
WB (1:200 - 1:2000)
Publication * U KP, Subramanian V et al. Modulation of calcium-induced cell death in human neural stem cells by the novel peptidylarginine deiminase-AIF pathway. Biochim Biophys Acta. 2014 Jun:1843(6):1162-71. (ICC, WB)
PADI2抗体文献参考:
* U KP, Subramanian V et al. Modulation of calcium-induced cell death in human neural stem cells by the novel peptidylarginine deiminase-AIF pathway. Biochim Biophys Acta. 2014 Jun:1843(6):1162-71. (ICC, WB)