waters液质tof 液相质谱重启流程

如何重新启动/开关电源包含 ACQUITY LC 的 Xevo Tof 系统 - WKB24861 目的 重新启动/开关电源包含 ACQUITY LC 的 Xevo Tof 系统。 环境 Xevo QTof Xevo G2 Xevo G2-S Xevo G2-XS 所有安装了 ACQUITY LC 的系统 步骤 将泵手动控制开关（即 Xevo 背面的底部中心位置两个切换开关中的上面一个）翻转到向下位置。您需要将开关拉出才能对其进行更改。然后，您应该能在 Tune 页面看到通知，显示泵手动控制处于激活状态。 退出 MassLynx 软件（关闭 Tune 页面、液相方法编辑器、控制台，然后关闭 MassLynx）。 运行 stopACQUITYProcesses（C:\Program files (x86)\Waters Instruments\Bin\StopACQUITYProcesses.cmd 或 StopProcesses.exe）。 重新启动采集计算机。 关闭 LC 模块电源。重要说明：关闭 LC 模块时，请确保连接以太网线缆的模块（通常为样品管理器）最后关闭。 关闭 LC 模块大约 30 秒。 重新打开 LC 模块。重要说明：请确保连接以太网线缆的模块最先打开。 如果是 Xevo G2，请关闭外部 EPC（所有其它仪器 EPC 将随电子线路关闭）。 向下翻转电子线路切换开关（Xevo 背面两个切换开关中的下面一个）。 等待大约 30 秒。 向上翻转 Xevo 背面的电子线路切换开关。 如果是 Xevo G2，请打开外部 EPC（所有其它仪器 EPC 将随电子线路打开）。 重要说明：等待 5 分钟，让 EPC 启动。 打开 MassLynx 软件。 打开 Tune 页面。 打开 View > Vacuum（查看 > 真空），然后打开真空选项卡。确认 Tof 的真空读数为真（非 1e-8）。如果未显示真实的 Tof 真空读数，请再次重新启动。 此外，还需要在 Tune 页面中查找错误信息，显示泵手动控制开关已处于手动控制位置。此错误信息表明，质谱仪和采集计算机之间的通讯正确。 显示良好的真空读数后，将泵手动控制开关（上面一个开关）翻转回自动（向上）位置。 确认第 17 步中记录的泵手动控制开关错误信息已从 Tune 页面消失。 将 MS 切换到 Operate（运行）模式 How to reboot and power-cycle a Xevo Tof system with ACQUITY LC - WKB24861 Last updatedDec 21, 2021 Save as PDF Share Article number: 24861 OBJECTIVE or GOAL Reboot and power-cycle a Xevo Tof system with ACQUITY LC. ENVIRONMENT Xevo QTof Xevo G2 Xevo G2-S Xevo G2-XS All with ACQUITY LC system attached PROCEDURE Flip the pump override switch (this is the top toggle switch of the two switches in the center at the the bottom on the back of the Xevo) to the down position. You need to pull the switch out to change it. You should then see a notification on the Tune Page that the pump override is active. Exit MassLynx (close the Tune Page, the Inlet Editor, the Console, and then MassLynx). Run stopACQUITYProcesses (C:\Program files (x86)\Waters Instruments\Bin\StopACQUITYProcesses.cmd or StopProcesses.exe). Reboot the acquisition computer. Power-down the LC stack. IMPORTANT: When powering-down the LC stack, ensure that the module with the Ethernet cables attached to it (most commonly the sample manager) is turned off last. Leave the LC stack off for approximately 30 seconds. Turn the LC stack back on. IMPORTANT: Ensure that the module with the Ethernet cables attached to it is turned on first. For a Xevo G2, power-off the external EPC (all other instrument EPCs power-off with the electronics). Flip the electronics toggle switch (the bottom switch of the two toggle switches on the back of the Xevo) down. Wait approximately 30 seconds. Flip the electronics toggle switch on the back of the Xevo up. For a Xevo G2, power-on the external EPC (all other instrument EPCs power-on with the electronics). IMPORTANT: Wait five minutes for the EPC to boot up. Open MassLynx. Open the Tune Page. Go to View > Vacuum, and then the vacuum tab. Verify that the vacuum reading for the Tof is real (not 1e-8). If there is no real Tof vacuum reading, start the reboot again. In the Tune Page, also look for an error message stating that the pump override switch is in the override position. This error message indicates that proper communication exists between the mass spectrometer and the acquisition computer. Once you have a good vacuum reading, flip the pump override switch (the top switch) back to the auto (up) position . Verify that the pump override switch error noted in step 17 is now gone from the Tune Page. Put the MS into Operate mode.