反馈有礼 | 好评之声！细胞因子美图赏析来了

细胞培养怎么离得开细胞因子？ABclonal提供不同种类的科研细胞因子，涉及生长因子、白介素、肿瘤坏死因子、趋化因子、集落刺激因子、干扰素等种类，可用于刺激细胞生长和分化、细胞信号转导以及涉及疾病发作和进展的研究，具有高纯度、高活性、低内毒素、低批间差的特点，助力广大科学研究者的工作！本期精选了一些细胞因子客户反馈美图供大家赏析。

RP01216

1.Active Recombinant Mouse CSF-1/M-CSF Protein

细胞：小鼠原代骨髓细胞

描述：Recombinant mouse M-CSF(20 ng/mL) induces mouse primary Bone Marrow cells to differentiate into Macrophages (BMDM). After 7 days, the induction was successful according to cell morphology.

客户评价：M-CSF可以有效诱导和维持骨髓巨噬细胞细胞形态。

RP01458

2.Active Recombinant Human Mature TGF-beta 1 Protein

细胞：MC7-F（人乳腺癌细胞）

图1

图2

描述：Active Recombinant Human Mature TGF-beta 1 induces MCF7 cells EMT with a concentration of 12.5 ng/mL. After 10 h, the cell morphology significantly altered, appearing a fusiform shape (figure 1). After 48 h, the change of EMT associated genes are as （figure 2）. Results showed that the induction was successful.

客户评价：细胞在诱导后的10H形态发生明显变化，呈现梭状，形态偏向于间充质细胞，发生EMT，但同时也会发生明显的细胞凋亡，可减少TGFB1的浓度，确定合适的诱导时间及诱导浓度。

RP01458

3.Active Recombinant Human Mature TGF-beta 1 Protein

细胞：HUVEC（人脐静脉内皮细胞）

描述：Measured by its ability to stimulate smad2/3 autophosphorylation in Human Umbilical Vein Endothelial Cells (HUVEC). 10ng/mL of Recombinant Human TGF-beta1 can effectively enhance smad2/3 autophosphorylation.

客户评价：刺激成功。

RP01161

4.Active Recombinant Mouse IL-4 Protein

细胞：C57BL/6J mouse bone marrow cells（C57BL/6J小鼠骨髓细胞）

描述：Recombinant mouse IL-4(10 ng/mL) and mouse GM-CSF(20 ng/mL) induce C57BL/6J mouse bone marrow cells to differentiate into BMDC. According to the Flowcytometry data, after 6 days, up to 75% of cells in the system expressed DC biomarker CD11C on cell surface, among which more than15% cells had high expression of MHCII. These data shows that the induction was successful.

客户评价：诱导成功。

RP01206 / RP01161

5.Active Recombinant Mouse CSF-2/GM-CSF Protein

Active Recombinant Mouse IL-4 Protein

细胞：C57BL/6小鼠骨髓细胞

描述：Recombinant Mouse IL-4 protein (10 ng/mL) and Mouse GM-CSF protein (20 ng/mL)induce C57BL/6 Mouse bone marrow cells to differentiate into BMDC. After 7 days, the BMDC biomarkers, CD11c, CD80 and CD86 on cell surface are 89.4%, 80.7% and 69.7% respectively.

客户评价：效果很好。

RP01070

6.Active Recombinant Mouse IFN-gamma Protein

细胞：BMDM（小鼠骨髓巨噬细胞）和PeritonealMacrophages（小鼠腹腔巨噬细胞）

描述：Recombinant mouse IFN-gamma stimulates mouse bone marrow-derived macrophages(Figure 1) and peritoneal macrophages (Figure 2) to differentiation into M1 Macrophages. The expression of related biomarkers TNF-α, Nos2, (IL-12), IL-1β, and IL-6 was detected to be significantly upregulated.

客户评价：相关标记物上调十分显著。

RP01345

7.Active Recombinant Mouse FGF-2/bFGF Protein

细胞：DPcell（真皮乳头细胞）

描述：Recombinant mouse FGF2 protein was added during DP cell culture. The immunofluorescence identification showed that 1 ng/mL FGF2 could maintain the cellular characteristics of DP cells. The red part is the fluorescently labeled PDFGRA antibody, and the blue-cyan part is the DAPI-stained cells.

客户评价：FGF2细胞因子可以使其维持真皮乳头的细胞特征。

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ABclonal

公司简介

爱博泰克生物科技有限公司（ABclonal Technology Co.,Ltd.）成立于2011年，作为生命科学解决方案的提供商，旨在为更多的科研工作者、IVD企业、新技术开发企业提供抗体、酶、NGS产品和蛋白制品等全套解决方案。