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重组荧光素酶,解决了天然来源的季节不稳定性和区域变异性

2023-02-21 15:52 作者:youngshe-Leslie  | 我要投稿

重组荧光素酶(rLUC)是一种从北美萤火虫(Photinus pyralis)克隆的基因表达的荧光素素酶,它提供了使用生物发光试剂检测ATP或荧光素基进行研究或生产试剂盒所需的可靠性和可靠性。重组来源消除了从天然来源纯化的荧光素酶中可能发现的季节性和区域性变异的可能性。

荧光素酶是一种发光的酶。多种生物利用荧光素酶介导的生物发光来惊吓捕食者或吸引猎物或配偶。北美萤火虫的荧光素酶在其化学底物荧光素的氧化过程中释放绿光。重组荧光素酶来源于大肠杆菌发酵。

Recombinant Luciferase is a luciferase  expressed from a cloned gene from the North American firefly (Photinus  pyralis) that provides the reliability and dependability needed for  performing research or producing kits using bioluminescence reagents to  detect ATP or luciferin substrates. A recombinant source eliminates the  possibility of seasonal and regional variability that may be found in  luciferase purified from natural sources.

Luciferases are enzymes that  emit light. A diverse group of organisms use luciferase-mediated   bioluminescence to startle predators or to attract prey or mates. The   luciferase from the North American fire fly releases green light during the oxidation of its chemical substrate, luciferin. Recombinant   luciferase is expressed inE. coli.


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