【标题速读】【Nprot】【2022年】【2-6月】
声明：本专栏主要对生命科学领域的一些期刊文章标题进行翻译，所有内容均由本人手工整理翻译。由于本人专业为生物分析相关，其他领域如果出现翻译错误请谅解。
多种人体组织的多重成像
Rainbow collage of IBEX images from nine different human tissues, including the lymph node, thymus, spleen, jejunum, kidney, liver, skin and heart. Individual images display unique cell types and anatomical structures defined by a single protein biomarker. All protein biomarkers are targeted by commercially available antibodies and obtained by IBEX, an open-source multiplexed imaging method.
来自九种不同人体组织的IBEX图像的彩虹拼贴画，包括淋巴结、胸腺、脾脏、空肠、肾脏、肝脏、皮肤和心脏。 单个图像显示由单个蛋白质生物标志物定义的独特细胞类型和解剖结构。所有蛋白质生物标志物均以市售抗体为目标，并通过开源多重成像方法IBEX获得。
1.Routine culture and study of adult human brain cells from neurosurgical specimens.
对来自神经外科标本的成年人类脑细胞进行常规培养和研究。
2.Neuronal subtype-specific growth cone and soma purification from mammalian CNS via fractionation and fluorescent sorting for subcellular analyses and spatial mapping of local transcriptomes and proteomes.
通过分馏和荧光分选从哺乳动物中枢神经系统纯化神经元亚型特异性生长锥和体细胞，用于亚细胞分析和局部转录组和蛋白质组的空间映射。
3.CRISPRi-seq for genome-wide fitness quantification in bacteria.
CRISPRi-seq用于细菌的全基因组适应性量化。
4.Designer DNA nanostructures for viral inhibition.
用于抑制病毒的DNA纳米结构的设计。
5.Protein transfection via spherical nucleic acids.
通过球形核酸的蛋白质转染。
6.High-yield production of mono- or few-layer transition metal dichalcogenide nanosheets by an electrochemical lithium ion intercalation-based exfoliation method.
基于电化学的锂离子插层剥离法高产生产单层或少层过渡金属二氯化物纳米片。
7.IBEX: an iterative immunolabeling and chemical bleaching method for high-content imaging of diverse tissues.
IBEX：一种迭代的免疫标记和化学漂白方法，用于不同组织的高含量成像。
8.KAS-seq: genome-wide sequencing of single-stranded DNA by N3-kethoxal–assisted labeling.
KAS-seq：通过N3-酮醇辅助标记对单链DNA进行全基因组测序。
9.High-resolution in situ structure determination by cryo-electron tomography and subtomogram averaging using emClarity.
使用emClarity通过低温电子断层扫描和子图平均化进行高分辨率原位结构测定。
10.Capture-C: a modular and flexible approach for high-resolution chromosome conformation capture.
Capture-C：一种用于高分辨率染色体构象捕获的模块化和灵活方法。
11.Pooled genetic perturbation screens with image-based phenotypes.
基于图像的表型的集合遗传扰动筛选。
12.Continuous human iPSC-macrophage mass production by suspension culture in stirred tank bioreactors.
在搅拌罐生物反应器中通过悬浮培养连续生产人类iPSC-巨噬细胞。
13.Stabilization and structure determination of integral membrane proteins by termini restraining.
通过端部抑制来稳定和确定整体膜蛋白的结构。
用FLARE染色的人乳腺组织
Formalin-fixed paraffin-embedded human breast tissue that was rapidly stained by FLARE (fluorescent labeling of abundant reactive entities), enabling informative visualization analogous to classic histology stains and holding the potential to revolutionize the clinical practice of examining tissue samples.
福尔马林固定石蜡包埋的人乳腺组织被FLARE（大量反应实体的荧光标记）快速染色，实现类似于经典组织学染色的信息可视化，并有可能彻底改变检查组织样本的临床实践。
1.Metabolic labeling of secreted matrix to investigate cell–material interactions in tissue engineering and mechanobiology.
分泌基质的代谢标记，研究组织工程和机械生物学中的细胞-材料相互作用。
2.Transplantation of intestinal organoids into a mouse model of colitis.
将肠道器官移植到小鼠结肠炎模型中。
3.Artificial intelligence–enabled virtual screening of ultra-large chemical libraries with deep docking.
人工智能驱动的超大型化学库的虚拟筛选与深度对接。
4.Universal platform for the generation of thermostabilized GPCRs that crystallize in LCP.
用于生成在LCP中结晶的热稳定GPCR的通用平台。
5.Quantum defects as versatile anchors for carbon nanotube functionalization.
量子缺陷作为碳纳米管功能化的多功能锚。
6.Synthesis of siRNA nanoparticles to silence plaque-destabilizing gene in atherosclerotic lesional macrophages.
合成siRNA纳米颗粒以沉默动脉粥样硬化病变巨噬细胞中的斑块稳定基因。
7.dCas9-VPR-mediated transcriptional activation of functionally equivalent genes for gene therapy.
dCas9-VPR介导的功能等同基因的转录激活用于基因治疗。
8.Fluorescent labeling of abundant reactive entities (FLARE) for cleared-tissue and super-resolution microscopy.
丰富的反应性实体的荧光标记（FLARE）用于干净的组织和超分辨率显微镜。
9.High-resolution imaging of bacterial spatial organization with vertical cell imaging by nanostructured immobilization (VerCINI).
通过纳米结构固定化的垂直细胞成像（VerCINI）对细菌空间组织进行高分辨成像。
10.Global mapping of RNA G-quadruplexes (G4-RNAs) using G4RP-seq.
使用G4RP-seq对RNA G-四重体（G4-RNAs）进行全球测绘。
11.Spotting-based differentiation of functional dopaminergic progenitors from human pluripotent stem cells.
基于斑点的人类多能干细胞功能多巴胺能祖细胞的分化。
12.3D in vitro morphogenesis of human intestinal epithelium in a gut-on-a-chip or a hybrid chip with a cell culture insert.
人类肠道上皮细胞在肠道芯片或带有细胞培养插件的混合芯片中的三维体外形态发生。
具有实时刺激控制的无线有源光遗传学装置
The layered schematics of a wireless optogenetic device capable of full subdermal implantation over the skull with real-time control over optical modulation on neural activities. Application of such devices in live rodent models enables profound behavior studies that explore the underlying neural principles behind outcome behaviors.
无线光遗传学设备的分层示意图，能够在头骨上完全皮下植入，实时控制神经活动的光学调制。在活的啮齿动物模型中应用此类设备可以进行深入的行为研究，探索结果行为背后的潜在神经原理。
1.Preparing ductal epithelial organoids for high-spatial-resolution molecular profiling using mass spectrometry imaging.
利用质谱成像技术为高空间分辨率的分子分析准备导管上皮细胞器官。
2.Synthesis of 68Ga-radiopharmaceuticals using both generator-derived and cyclotron-produced 68Ga as exemplified by [68Ga]Ga-PSMA-11 for prostate cancer PET imaging.
以用于前列腺癌PET成像的[68Ga]Ga-PSMA-11为例，利用发生器产生的和回旋加速器产生的68Ga合成68Ga放射性药物。
3.Generation of CRISPR–Cas9-mediated genetic knockout human intestinal tissue–derived enteroid lines by lentivirus transduction and single-cell cloning.
通过慢病毒转导和单细胞克隆产生CRISPR-Cas9介导的基因敲除人类肠道组织衍生的肠系。
4.Bioconjugates of photon-upconversion nanoparticles for cancer biomarker detection and imaging.
用于癌症生物标志物检测和成像的光子上转换纳米粒子的生物结合物。
5.Preparation and use of wireless reprogrammable multilateral optogenetic devices for behavioral neuroscience.
用于行为神经科学的无线可重复编程多边光遗传装置的制备和使用。
6.Genetic circuit design automation with Cello 2.0.
用Cello 2.0实现遗传电路设计自动化。
7.Accurate determination of protein:ligand standard binding free energies from molecular dynamics simulations.
从分子动力学模拟中精确确定蛋白质：配体标准结合自由能。
8.CRISPR somatic genome engineering and cancer modeling in the mouse pancreas and liver.
小鼠胰腺和肝脏的CRISPR体细胞基因组工程和癌症模型的建立。
有毛发的皮肤类器官
The image displays a human-stem-cell-derived skin organoid with radially growing hair follicles (green) and nerves (red/yellow). This particular organoid was grown from a genetically modified stem cell line where the Desmoplakin gene/protein is tagged with a GFP. As a consequence, the numerous desmosomes (cell–cell junctions) in the epidermis of the organoid glow green. Neurons are labeled with an antibody for beta-III tubulin.
该图像显示了人类干细胞衍生的皮肤类器官，具有径向生长的毛囊（绿色）和神经（红色/黄色）。这种特殊的类器官是从转基因干细胞系中生长出来的，其中Desmoplakin基因/蛋白被GFP标记。因此，类器官表皮中的大量桥粒（细胞-细胞连接）发出绿色光。神经元用β-III微管蛋白抗体标记。
1.Transcriptome-wide identification of RNA-binding protein binding sites using seCLIP-seq.
利用seCLIP-seq在整个转录组中鉴定RNA结合蛋白的结合点。
2.Generation and characterization of hair-bearing skin organoids from human pluripotent stem cells.
来自人类多能干细胞的毛发皮肤器官的生成和特征。
3.RASER-FISH: non-denaturing fluorescence in situ hybridization for preservation of three-dimensional interphase chromatin structure.
RASER-FISH：非变性荧光原位杂交技术保存三维间期染色质结构。
4.CRISPR–Cas9-mediated chromosome engineering in Arabidopsis thaliana.
CRISPR-Cas9介导的拟南芥染色体工程。
5.Isolation of mouse pancreatic islet Procr+ progenitors and long-term expansion of islet organoids in vitro.
小鼠胰岛Procr+祖细胞的分离和体外胰岛器官的长期扩增。
表征细胞类型特异性染色质景观
ATAC-seq profiles the gene regulatory ‘landscape’ of a cell. Promoters, enhancers and other putative gene regulatory elements are identified as peaks in the data and these signals are highly cell-type-specific. Here, the skylines of Paris, San Francisco, New York City and Shanghai are depicted in the style of cell-type-specific ATAC-seq peaks.
ATAC-seq描述了细胞的基因调控“景观”。启动子、增强子和其他假定的基因调控元件被识别为数据中的峰值，这些信号具有高度的细胞类型特异性。在这里，巴黎、旧金山、纽约市和上海的天际线以细胞类型特定的ATAC-seq峰的风格描绘。
1.Rapid in situ identification of biological specimens via DNA amplicon sequencing using miniaturized laboratory equipment.
利用小型化实验室设备通过DNA扩增子测序对生物标本进行快速原位鉴定。
2.Purification of mammalian telomeric DNA for single-molecule analysis.
纯化哺乳动物端粒DNA进行单分子分析。
3.Subretinal injection in mice to study retinal physiology and disease.
小鼠视网膜下注射，研究视网膜的生理和疾病。
4.Sister chromatid–sensitive Hi-C to map the conformation of replicated genomes.
姐妹染色体敏感的Hi-C来绘制复制基因组的构象。
5.Chromatin accessibility profiling by ATAC-seq.
通过ATAC-seq进行染色质可及性分析。
