Research Grade Anti-RSV F/Fusion glycoprotein F0 (F4)

RSV是一种反式单链RNA病毒，共编码11种蛋白，其中G和F表面蛋白在RSV吸附和融合过程中发挥重要作用，是产生中和抗体最重要的抗原位点，也是诱导机体产生免疫原性和抗病毒的最主要靶点。

货号：DVV02823

产品链接：http://www.antibodysystem.com/product/9576.html

物种反应性：Human respiratory syncytial virus A (strain A2)

形式：Liquid

存储缓冲区：0.01M PBS, pH 7.4.

浓度：1 mg/ml

纯度：>95% by SDS-PAGE.

克隆性：Monoclonal

应用：Research Grade Biosimilar

储存：Use a manual defrost freezer and avoid repeated freeze-thaw cycles.Store at +4°C short term (1-2 weeks).Store at -20 °C 12 months. Store at -80°C long term.

参考文献：

F4/80 as a Major Macrophage Marker: The Case of the Peritoneum and Spleen. PMID: 28455709
F4/80: the macrophage-specific adhesion-GPCR and its role in immunoregulation. PMID: 21618834
F4/80 and the related adhesion-GPCRs. PMID: 21952799
The F4 fimbrial antigen of Escherichia coli and its receptors. PMID: 10703706
Receptor for the F4 fimbriae of enterotoxigenic Escherichia coli (ETEC). PMID: 25967654
Methodology and application of Escherichia coli F4 and F18 encoding infection models in post-weaning pigs. PMID: 31210932
F4-TCNQ on Epitaxial Bi-Layer Graphene: Concentration- and Orientation-Dependent Charge Transfer at the Interface. PMID: 36512752
F4, a collagen XIX-derived peptide, inhibits tumor angiogenesis through αvβ3 and α5β1 integrin interaction. PMID: 34308743
A macrophage-endothelial immunoregulatory axis ameliorates septic acute kidney injury. PMID: 36334787
分别构建携带RSV-B1株F基因截短片段和全长的重组腺病毒rAd-F0DeltaTM和rAd-F0。在BALB/c小鼠中进行的免疫原性比较研究表明，每种载体均能诱导产生与RSV-long和RSV-A2病毒交叉反应的rsv - b1特异性抗体。抗rsv - b1抗体具有中和活性，对rsv -长株和RSV-A2分离株也具有较强的交叉中和活性。细胞免疫反应分析显示，rAd-F0DeltaTM和rAd-F0均能诱导Th1和Th2表型的CD4(+) t细胞应答和F蛋白特异性ctl。rAd-F0DeltaTM和rAd-F0免疫小鼠脾细胞产生的Th2细胞因子(IL-4、IL-5和IL-13)明显低于热灭活RSV-B1 (hirv - b1)免疫小鼠。总体体液和细胞免疫应答的比较表明，rAd-F0DeltaTM较rAd-F0具有更强的免疫原性。与接种hirv - b1的小鼠相比，两种重组腺病毒载体产生的抗病毒免疫对RSV-B1活病毒攻击产生了保护作用，原因是肺内恢复的病毒载量较低，体重减轻的恢复速度较快，嗜酸性粒细胞计数较低。这些研究结果表明，rAd-F0DeltaTM或rAd-F0具有符合RSV候选疫苗预期要求的免疫原性。