ESIPT荧光探针
ESIPT荧光探针（ESIPT fluorescent probe）是一种对蛋白α螺旋结构敏感的荧光探针，ESIPT荧光探针 用以区分单个前列腺癌细胞和正常前列腺癌细胞。该探针在蛋白α-螺旋环境中激发态分子内质子转移（ESIPT）产生强荧光.
溶解度SolubilitySoluble in water
性状Solid power
储藏条件 Storage conditionsstorage at -4℃ (1-2weeks), longer storage period at -20℃ (1-2years)
引用文献：
A large majority of membrane proteins have one or more transmembrane regions consisting of α-helices. Membrane protein levels differ from one type of cell to another, and the expression of membrane proteins also changes from normal to diseased cells. For example, prostate cancer cells have been reported to have downregulated expression of membrane proteins, including zinc transporters, compared with normal prostate cells. These reports inspired us to design a fluorescence probe sensitive to protein α-helical structures to discriminate individual prostate cancer cells from normal ones. A benzazole derivative (1 in this study) was observed to emit strong fluorescence resulting from an excited-state intramolecular proton transfer (ESIPT) in protein α-helical environments. The intensity of ESIPT fluorescence of 1 was observed to be positively correlated with the α-helix content of proteins. The molecular docking simulation suggested that it had low energy for the binding of 1 to proteins when the binding sites were localized within the α-helical regions of protein via H-bonds. Furthermore, 1 was found to be localized in cell membranes through binding to transmembrane α-helical regions of membrane proteins, and was capable of probing differences in the α-helix contents of membrane proteins between normal and cancerous prostate cells through changes in the ESIPT emission intensity. These results indicated that 1 could distinguish individual prostate cancer cells from normal ones, as the changes in the ESIPT fluorescence intensity of 1 could reflect the regulation in expression of the membrane proteins including zinc transporters. This recognition strategy of individual prostate cancer cells might contribute to early diagnosis techniques for prostate cancer.
